Assay Detail
Binding
CHEMBL5730833
Review assay metadata, readout intent, target linkage, and publication context from the same page.
Radiochemistry Enzymatic Assay: A human VAP-1 enzyme (SSAO) (reference: J Exp Med. 1998 Jul. 6; 188(1): 17 to 27) activity was measured by a radiochemistry-enzymatic assay using 14C-benzylamine as an artificial substrate. After homogenizing CHO (Chinese Hamster Ovary) cells stably expressing a human VAP-1 enzyme (SSAO) in a 50 mM phosphate buffer containing 1% NP-40, an enzyme suspension was obtained by taking the supernatant obtained by centrifugation. The enzyme suspension was preincubated with the compound of the present invention in a 96-well microplate at room temperature for 30 minutes. Subsequently, the enzyme suspension was incubated with 14C-benzylamine (a final concentration of 1×10−5 mol/L) to a final volume of 50 mL at 37° C. for 1 hour. The enzymatic reaction was stopped by the addition of 2 mol/L (50 pt) of citric acid. The oxidation products were extracted directly into a 2004, toluene scintillator, and the radioactivity was measured with a scintillation spectrometer.
114
Total Activities
37
Compounds Tested
3
Activity Types
0
Assay Parameters
Assay Information
| Assay Type | Binding |
| Organism | Homo sapiens |
| Confidence | 9 — Direct single protein target |
| Curated By | Autocuration |
Publication
Guanidine compound
Activity Statistics
| Type | Count | Avg pChEMBL | Best pChEMBL |
|---|---|---|---|
| IC50 | 38 | 7.84 | 8.57 |
| kon | 38 | - | - |
| k_off | 38 | - | - |
Compounds Tested
| Compound | Name | Phase | Activities | Best pChEMBL |
|---|---|---|---|---|
| CHEMBL5741414 | — | — | 3 | 8.57 |
| CHEMBL6011649 | — | — | 3 | 8.47 |
| CHEMBL5816555 | — | — | 6 | 8.37 |
| CHEMBL5807211 | — | — | 3 | 8.28 |
| CHEMBL5996897 | — | — | 3 | 8.28 |
| CHEMBL5815180 | — | — | 3 | 8.20 |
| CHEMBL5951266 | — | — | 3 | 8.14 |
| CHEMBL6045381 | — | — | 3 | 8.05 |
| CHEMBL5847433 | — | — | 3 | 8.02 |
| CHEMBL6020987 | — | — | 3 | 8.01 |
| CHEMBL5877970 | — | — | 3 | 7.96 |
| CHEMBL5764728 | — | — | 3 | 7.96 |
| CHEMBL6014613 | — | — | 3 | 7.96 |
| CHEMBL6034079 | — | — | 3 | 7.85 |
| CHEMBL5929646 | — | — | 3 | 7.85 |
| CHEMBL6014894 | — | — | 3 | 7.85 |
| CHEMBL5892320 | — | — | 3 | 7.82 |
| CHEMBL5785705 | — | — | 3 | 7.72 |
| CHEMBL5940131 | — | — | 3 | 7.72 |
| CHEMBL5753110 | — | — | 3 | 7.72 |
| CHEMBL5776825 | — | — | 3 | 7.72 |
| CHEMBL5838951 | — | — | 3 | 7.72 |
| CHEMBL5814853 | — | — | 3 | 7.70 |
| CHEMBL5757911 | — | — | 3 | 7.70 |
| CHEMBL5985341 | — | — | 3 | 7.70 |
| CHEMBL6014263 | — | — | 3 | 7.68 |
| CHEMBL6018287 | — | — | 3 | 7.64 |
| CHEMBL5957502 | — | — | 3 | 7.64 |
| CHEMBL5917130 | — | — | 3 | 7.60 |
| CHEMBL5756303 | — | — | 3 | 7.60 |
Activity Data
| Compound | Name | Type | Rel. | Value | Units | pChEMBL |
|---|---|---|---|---|---|---|
| CHEMBL5741414 | — | IC50 | = | 2.7 | nM | 8.57 |
| CHEMBL6011649 | — | IC50 | = | 3.4 | nM | 8.47 |
| CHEMBL5816555 | — | IC50 | = | 4.3 | nM | 8.37 |
| CHEMBL5816555 | — | IC50 | = | 4.3 | nM | 8.37 |
| CHEMBL5807211 | — | IC50 | = | 5.2 | nM | 8.28 |
| CHEMBL5996897 | — | IC50 | = | 5.3 | nM | 8.28 |
| CHEMBL5815180 | — | IC50 | = | 6.3 | nM | 8.20 |
| CHEMBL5951266 | — | IC50 | = | 7.2 | nM | 8.14 |
| CHEMBL6045381 | — | IC50 | = | 9.0 | nM | 8.05 |
| CHEMBL5847433 | — | IC50 | = | 9.6 | nM | 8.02 |
| CHEMBL6020987 | — | IC50 | = | 9.7 | nM | 8.01 |
| CHEMBL5764728 | — | IC50 | = | 11.0 | nM | 7.96 |
| CHEMBL6014613 | — | IC50 | = | 11.0 | nM | 7.96 |
| CHEMBL5877970 | — | IC50 | = | 11.0 | nM | 7.96 |
| CHEMBL6034079 | — | IC50 | = | 14.0 | nM | 7.85 |
| CHEMBL6014894 | — | IC50 | = | 14.0 | nM | 7.85 |
| CHEMBL5929646 | — | IC50 | = | 14.0 | nM | 7.85 |
| CHEMBL5892320 | — | IC50 | = | 15.0 | nM | 7.82 |
| CHEMBL5940131 | — | IC50 | = | 19.0 | nM | 7.72 |
| CHEMBL5753110 | — | IC50 | = | 19.0 | nM | 7.72 |
| CHEMBL5776825 | — | IC50 | = | 19.0 | nM | 7.72 |
| CHEMBL5785705 | — | IC50 | = | 19.0 | nM | 7.72 |
| CHEMBL5838951 | — | IC50 | = | 19.0 | nM | 7.72 |
| CHEMBL5814853 | — | IC50 | = | 20.0 | nM | 7.70 |
| CHEMBL5757911 | — | IC50 | = | 20.0 | nM | 7.70 |
| CHEMBL5985341 | — | IC50 | = | 20.0 | nM | 7.70 |
| CHEMBL6014263 | — | IC50 | = | 21.0 | nM | 7.68 |
| CHEMBL6018287 | — | IC50 | = | 23.0 | nM | 7.64 |
| CHEMBL5957502 | — | IC50 | = | 23.0 | nM | 7.64 |
| CHEMBL5917130 | — | IC50 | = | 25.0 | nM | 7.60 |
| CHEMBL5756303 | — | IC50 | = | 25.0 | nM | 7.60 |
| CHEMBL5915647 | — | IC50 | = | 25.0 | nM | 7.60 |
| CHEMBL5909475 | — | IC50 | = | 29.0 | nM | 7.54 |
| CHEMBL5872917 | — | IC50 | = | 32.0 | nM | 7.50 |
| CHEMBL5778239 | — | IC50 | = | 33.0 | nM | 7.48 |
| CHEMBL5772276 | — | IC50 | = | 34.0 | nM | 7.47 |
| CHEMBL5993668 | — | IC50 | = | 49.0 | nM | 7.31 |
| CHEMBL5909531 | — | IC50 | = | 51.0 | nM | 7.29 |
| CHEMBL5816555 | — | k_off | = | - | s-1 | - |
| CHEMBL5816555 | — | kon | = | - | — | - |
| CHEMBL5741414 | — | kon | = | - | — | - |
| CHEMBL5940131 | — | k_off | = | - | s-1 | - |
| CHEMBL5815180 | — | kon | = | - | — | - |
| CHEMBL5985341 | — | k_off | = | - | s-1 | - |
| CHEMBL5985341 | — | kon | = | - | — | - |
| CHEMBL6014263 | — | k_off | = | - | s-1 | - |
| CHEMBL6014263 | — | kon | = | - | — | - |
| CHEMBL5815180 | — | k_off | = | - | s-1 | - |
| CHEMBL5929646 | — | kon | = | - | — | - |
| CHEMBL5757911 | — | k_off | = | - | s-1 | - |