Assay Detail
Binding
CHEMBL5730834
Review assay metadata, readout intent, target linkage, and publication context from the same page.
Radiochemistry Enzymatic Assay: A rat VAP-1 enzyme (SSAO) (reference: Biol Pharm Bull. 2005 March; 28(3): 413-8) activity was measured by a radiochemistry-enzymatic assay using 14C-benzylamine as an artificial substrate. After homogenizing CHO (Chinese Hamster Ovary) cells stably expressing a rat VAP-1 enzyme (SSAO) in a 50 mM phosphate buffer containing 1% NP-40, an enzyme suspension was obtained by taking the supernatant obtained by centrifugation. The enzyme suspension was preincubated with the compound of the present invention in a 96-well microplate at room temperature for 30 minutes. Subsequently, the enzyme suspension was incubated with 14C-benzylamine (a final concentration of 1×10−5 mol/L) to a final volume of 50 mL at 37° C. for 1 hour. The enzymatic reaction was stopped by the addition of 2 mol/L (50 μL) of citric acid. The oxidation products were extracted directly in a 200 μL toluene scintillator, and the radioactivity was measured with a scintillation spectrometer.
114
Total Activities
37
Compounds Tested
3
Activity Types
0
Assay Parameters
Assay Information
| Assay Type | Binding |
| Confidence | 8 — Homologous single protein target |
| Curated By | Autocuration |
Target
Amine oxidase [copper-containing] 3 (CHEMBL4592) ↗| Type | SINGLE PROTEIN |
| Organism | Rattus norvegicus |
Publication
Guanidine compound
Activity Statistics
| Type | Count | Avg pChEMBL | Best pChEMBL |
|---|---|---|---|
| IC50 | 38 | 8.75 | 9.28 |
| kon | 38 | - | - |
| k_off | 38 | - | - |
Compounds Tested
| Compound | Name | Phase | Activities | Best pChEMBL |
|---|---|---|---|---|
| CHEMBL5892320 | — | — | 3 | 9.28 |
| CHEMBL5816555 | — | — | 6 | 9.09 |
| CHEMBL6014613 | — | — | 3 | 9.05 |
| CHEMBL5838951 | — | — | 3 | 9.02 |
| CHEMBL6014263 | — | — | 3 | 9.01 |
| CHEMBL5776825 | — | — | 3 | 9.01 |
| CHEMBL5915647 | — | — | 3 | 9.00 |
| CHEMBL6011649 | — | — | 3 | 8.96 |
| CHEMBL5815180 | — | — | 3 | 8.92 |
| CHEMBL6018287 | — | — | 3 | 8.92 |
| CHEMBL5741414 | — | — | 3 | 8.89 |
| CHEMBL5872917 | — | — | 3 | 8.85 |
| CHEMBL6045381 | — | — | 3 | 8.85 |
| CHEMBL5929646 | — | — | 3 | 8.85 |
| CHEMBL5814853 | — | — | 3 | 8.82 |
| CHEMBL5785705 | — | — | 3 | 8.82 |
| CHEMBL5847433 | — | — | 3 | 8.80 |
| CHEMBL5764728 | — | — | 3 | 8.80 |
| CHEMBL5753110 | — | — | 3 | 8.77 |
| CHEMBL5957502 | — | — | 3 | 8.77 |
| CHEMBL5909475 | — | — | 3 | 8.77 |
| CHEMBL6034079 | — | — | 3 | 8.74 |
| CHEMBL5996897 | — | — | 3 | 8.74 |
| CHEMBL5756303 | — | — | 3 | 8.72 |
| CHEMBL5951266 | — | — | 3 | 8.72 |
| CHEMBL5877970 | — | — | 3 | 8.70 |
| CHEMBL5993668 | — | — | 3 | 8.68 |
| CHEMBL5807211 | — | — | 3 | 8.66 |
| CHEMBL5940131 | — | — | 3 | 8.60 |
| CHEMBL6014894 | — | — | 3 | 8.59 |
Activity Data
| Compound | Name | Type | Rel. | Value | Units | pChEMBL |
|---|---|---|---|---|---|---|
| CHEMBL5892320 | — | IC50 | = | 0.53 | nM | 9.28 |
| CHEMBL5816555 | — | IC50 | = | 0.81 | nM | 9.09 |
| CHEMBL5816555 | — | IC50 | = | 0.81 | nM | 9.09 |
| CHEMBL6014613 | — | IC50 | = | 0.9 | nM | 9.05 |
| CHEMBL5838951 | — | IC50 | = | 0.95 | nM | 9.02 |
| CHEMBL6014263 | — | IC50 | = | 0.97 | nM | 9.01 |
| CHEMBL5776825 | — | IC50 | = | 0.97 | nM | 9.01 |
| CHEMBL5915647 | — | IC50 | = | 1.0 | nM | 9.00 |
| CHEMBL6011649 | — | IC50 | = | 1.1 | nM | 8.96 |
| CHEMBL5815180 | — | IC50 | = | 1.2 | nM | 8.92 |
| CHEMBL6018287 | — | IC50 | = | 1.2 | nM | 8.92 |
| CHEMBL5741414 | — | IC50 | = | 1.3 | nM | 8.89 |
| CHEMBL5929646 | — | IC50 | = | 1.4 | nM | 8.85 |
| CHEMBL6045381 | — | IC50 | = | 1.4 | nM | 8.85 |
| CHEMBL5872917 | — | IC50 | = | 1.4 | nM | 8.85 |
| CHEMBL5785705 | — | IC50 | = | 1.5 | nM | 8.82 |
| CHEMBL5814853 | — | IC50 | = | 1.5 | nM | 8.82 |
| CHEMBL5764728 | — | IC50 | = | 1.6 | nM | 8.80 |
| CHEMBL5847433 | — | IC50 | = | 1.6 | nM | 8.80 |
| CHEMBL5957502 | — | IC50 | = | 1.7 | nM | 8.77 |
| CHEMBL5753110 | — | IC50 | = | 1.7 | nM | 8.77 |
| CHEMBL5909475 | — | IC50 | = | 1.7 | nM | 8.77 |
| CHEMBL6034079 | — | IC50 | = | 1.8 | nM | 8.74 |
| CHEMBL5996897 | — | IC50 | = | 1.8 | nM | 8.74 |
| CHEMBL5756303 | — | IC50 | = | 1.9 | nM | 8.72 |
| CHEMBL5951266 | — | IC50 | = | 1.9 | nM | 8.72 |
| CHEMBL5877970 | — | IC50 | = | 2.0 | nM | 8.70 |
| CHEMBL5993668 | — | IC50 | = | 2.1 | nM | 8.68 |
| CHEMBL5807211 | — | IC50 | = | 2.2 | nM | 8.66 |
| CHEMBL5940131 | — | IC50 | = | 2.5 | nM | 8.60 |
| CHEMBL6014894 | — | IC50 | = | 2.6 | nM | 8.59 |
| CHEMBL5757911 | — | IC50 | = | 3.0 | nM | 8.52 |
| CHEMBL5985341 | — | IC50 | = | 3.5 | nM | 8.46 |
| CHEMBL6020987 | — | IC50 | = | 3.9 | nM | 8.41 |
| CHEMBL5778239 | — | IC50 | = | 4.4 | nM | 8.36 |
| CHEMBL5917130 | — | IC50 | = | 5.5 | nM | 8.26 |
| CHEMBL5772276 | — | IC50 | = | 6.6 | nM | 8.18 |
| CHEMBL5909531 | — | IC50 | = | 22.0 | nM | 7.66 |
| CHEMBL6014263 | — | k_off | = | - | s-1 | - |
| CHEMBL5741414 | — | kon | = | - | — | - |
| CHEMBL5940131 | — | kon | = | - | — | - |
| CHEMBL5940131 | — | k_off | = | - | s-1 | - |
| CHEMBL5985341 | — | k_off | = | - | s-1 | - |
| CHEMBL5985341 | — | kon | = | - | — | - |
| CHEMBL5816555 | — | kon | = | - | — | - |
| CHEMBL5816555 | — | k_off | = | - | s-1 | - |
| CHEMBL5757911 | — | kon | = | - | — | - |
| CHEMBL5757911 | — | k_off | = | - | s-1 | - |
| CHEMBL6014263 | — | kon | = | - | — | - |
| CHEMBL5815180 | — | kon | = | - | — | - |