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Assay Detail

CHEMBL5732258

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Electrophysiological Assay : The following patch voltage clamp electrophysiology studies were performed on representative compounds of the invention using human embryonic kidney cells (HEK), permanently transfected with an expression vector containing the full-length cDNA coding for the desired human sodium channel α-subunit, grown in culture media containing 10% FBS, 1% PSG, and 0.5 mg/mL G418 at 37° C. with 5% CO2. HEK cells used for the electrophysiology (EP) recordings had a passage number of less than 40 for all studies and were used within three days from the time of plating. Nav1.7 and Nav1.5 cDNAs (NM_002977 and AC137587; SCN5A, respectively) were stably expressed in HEK-293 cells. The β1 subunit was coexpressed only in the Nav1.7 cell line.
288
Total Activities
57
Compounds Tested
3
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Confidence 0 — Uncurated / Unknown
Curated By Autocuration

Target

Unchecked (CHEMBL612545)
Type UNCHECKED

Publication

Benzenesulfonamide compounds and their use as therapeutic agents
(2017)

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 96 6.85 9.10
kon 96 - -
k_off 96 - -

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL5781390 6 9.10
CHEMBL5897347 6 9.05
CHEMBL3325526 6 8.96
CHEMBL6011175 6 8.59
CHEMBL5982736 12 8.48
CHEMBL5766935 6 8.48
CHEMBL3325537 6 8.32
CHEMBL5803464 6 8.17
CHEMBL6028339 6 8.07
CHEMBL5807668 6 7.92
CHEMBL5797625 6 7.67
CHEMBL6051990 6 7.64
CHEMBL5847974 6 7.50
CHEMBL5934221 6 7.48
CHEMBL5833824 6 7.42
CHEMBL5943670 6 7.42
CHEMBL5945564 6 7.30
CHEMBL3325530 6 7.29
CHEMBL5859656 6 7.27
CHEMBL5872027 6 7.15
CHEMBL5804891 6 7.12
CHEMBL5908065 6 7.09
CHEMBL5967832 6 7.07
CHEMBL5742819 6 7.03
CHEMBL5876192 6 6.94
CHEMBL5853880 6 6.89
CHEMBL5962861 3 6.88
CHEMBL5801875 6 6.79
CHEMBL5989696 6 6.77
CHEMBL5760755 3 6.74

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL5781390 IC50 = 0.8 nM 9.10
CHEMBL5897347 IC50 = 0.9 nM 9.05
CHEMBL3325526 IC50 = 1.1 nM 8.96
CHEMBL6011175 IC50 = 2.6 nM 8.59
CHEMBL5982736 IC50 = 3.3 nM 8.48
CHEMBL5766935 IC50 = 3.3 nM 8.48
CHEMBL3325537 IC50 = 4.8 nM 8.32
CHEMBL5803464 IC50 = 6.8 nM 8.17
CHEMBL6028339 IC50 = 8.6 nM 8.07
CHEMBL5807668 IC50 = 12.0 nM 7.92
CHEMBL5797625 IC50 = 21.6 nM 7.67
CHEMBL6051990 IC50 = 23.0 nM 7.64
CHEMBL5847974 IC50 = 31.7 nM 7.50
CHEMBL5934221 IC50 = 33.0 nM 7.48
CHEMBL5833824 IC50 = 38.1 nM 7.42
CHEMBL5943670 IC50 = 38.0 nM 7.42
CHEMBL5945564 IC50 = 49.9 nM 7.30
CHEMBL3325530 IC50 = 50.7 nM 7.29
CHEMBL5859656 IC50 = 53.2 nM 7.27
CHEMBL5982736 IC50 = 58.8 nM 7.23
CHEMBL5872027 IC50 = 70.5 nM 7.15
CHEMBL5943670 IC50 = 71.0 nM 7.15
CHEMBL5804891 IC50 = 76.4 nM 7.12
CHEMBL5908065 IC50 = 82.0 nM 7.09
CHEMBL5967832 IC50 = 84.4 nM 7.07
CHEMBL5742819 IC50 = 93.7 nM 7.03
CHEMBL5876192 IC50 = 116.0 nM 6.94
CHEMBL5853880 IC50 = 130.0 nM 6.89
CHEMBL5962861 IC50 = 132.0 nM 6.88
CHEMBL5801875 IC50 = 164.0 nM 6.79
CHEMBL5989696 IC50 = 171.0 nM 6.77
CHEMBL5760755 IC50 = 181.0 nM 6.74
CHEMBL6006694 IC50 = 194.0 nM 6.71
CHEMBL5805300 IC50 = 237.0 nM 6.62
CHEMBL5861673 IC50 = 244.0 nM 6.61
CHEMBL5996787 IC50 = 280.0 nM 6.55
CHEMBL5765046 IC50 = 326.0 nM 6.49
CHEMBL5983136 IC50 = 323.0 nM 6.49
CHEMBL3325536 IC50 = 331.0 nM 6.48
CHEMBL5879530 IC50 = 393.0 nM 6.41
CHEMBL5804743 IC50 = 426.0 nM 6.37
CHEMBL5991925 IC50 = 436.0 nM 6.36
CHEMBL5801875 IC50 = 550.0 nM 6.26
CHEMBL5876192 IC50 = 550.0 nM 6.26
CHEMBL6006694 IC50 = 550.0 nM 6.26
CHEMBL5996787 IC50 = 550.0 nM 6.26
CHEMBL5853880 IC50 = 550.0 nM 6.26
CHEMBL3325536 IC50 = 550.0 nM 6.26
CHEMBL5805300 IC50 = 550.0 nM 6.26
CHEMBL5989696 IC50 = 550.0 nM 6.26