Assay Detail
Binding
CHEMBL5732491
Review assay metadata, readout intent, target linkage, and publication context from the same page.
Enzyme Inhibition Assay: The inhibitory activities of compounds of the invention against the GSK 3α enzyme isoform (Invitrogen), are evaluated by determining the level of activation/phosphorylation of the target peptide. The GSK3-α protein (500 ng/mL, 2.5 μL) is mixed with the test compound (2.5 μL at either 4 μg/mL, 0.4 μg/mL, 0.04 μg/mL, or 0.004 μg/mL) for 2 hr at RT. The FRET peptide (8 μM, 2.5 μL), which is a phosphorylation target for GSK3α, and ATP (40 μM, 2.5 μL) are then added to the enzyme/compound mixture and the resulting mixture incubated for 1 hr. Development reagent (protease, 5 μL) is added for 1 hr prior to detection in a fluorescence microplate reader (Varioskan® Flash, ThermoFisher Scientific).In all cases, the site-specific protease cleaves non-phosphorylated peptide only and eliminates the FRET signal. Phosphorylation levels of each reaction are calculated using the ratio of coumarin emission (donor) over fluorescein emission (acceptor), for which high ratios indicate high phosphorylation and low ratios indicate low phosphorylation levels.
216
Total Activities
72
Compounds Tested
3
Activity Types
0
Assay Parameters
Assay Information
| Assay Type | Binding |
| Organism | Homo sapiens |
| Confidence | 9 — Direct single protein target |
| Curated By | Autocuration |
Publication
Kinase inhibitors
Activity Statistics
| Type | Count | Avg pChEMBL | Best pChEMBL |
|---|---|---|---|
| IC50 | 72 | 5.72 | 6.93 |
| kon | 72 | - | - |
| k_off | 72 | - | - |
Compounds Tested
| Compound | Name | Phase | Activities | Best pChEMBL |
|---|---|---|---|---|
| CHEMBL5912287 | — | — | 3 | 6.93 |
| CHEMBL5866923 | — | — | 3 | 6.90 |
| CHEMBL5992634 | — | — | 3 | 6.76 |
| CHEMBL5753503 | — | — | 3 | 6.59 |
| CHEMBL6046794 | — | — | 3 | 6.51 |
| CHEMBL6016170 | — | — | 3 | 6.38 |
| CHEMBL5990752 | — | — | 3 | 6.37 |
| CHEMBL5976851 | — | — | 3 | 6.30 |
| CHEMBL6023399 | — | — | 3 | 6.28 |
| CHEMBL5954666 | — | — | 3 | 6.22 |
| CHEMBL5827694 | — | — | 3 | 6.09 |
| CHEMBL5979374 | — | — | 3 | 6.08 |
| CHEMBL5858089 | — | — | 3 | 6.01 |
| CHEMBL5991930 | — | — | 3 | 5.99 |
| CHEMBL5935125 | — | — | 3 | 5.97 |
| CHEMBL5802500 | — | — | 3 | 5.91 |
| CHEMBL5820101 | — | — | 3 | 5.88 |
| CHEMBL5855478 | — | — | 3 | 5.85 |
| CHEMBL6030562 | — | — | 3 | 5.83 |
| CHEMBL6040369 | — | — | 3 | 5.73 |
| CHEMBL5752694 | — | — | 3 | 5.71 |
| CHEMBL5919556 | — | — | 3 | 5.71 |
| CHEMBL5841040 | — | — | 3 | 5.69 |
| CHEMBL5933911 | — | — | 3 | 5.60 |
| CHEMBL5968094 | — | — | 3 | 5.57 |
| CHEMBL5753333 | — | — | 3 | 5.55 |
| CHEMBL5823917 | — | — | 3 | 5.55 |
| CHEMBL5872801 | — | — | 3 | 5.54 |
| CHEMBL5873953 | — | — | 3 | 5.52 |
| CHEMBL5928253 | — | — | 3 | 5.47 |
Activity Data
| Compound | Name | Type | Rel. | Value | Units | pChEMBL |
|---|---|---|---|---|---|---|
| CHEMBL5912287 | — | IC50 | = | 117.0 | nM | 6.93 |
| CHEMBL5866923 | — | IC50 | = | 125.0 | nM | 6.90 |
| CHEMBL5992634 | — | IC50 | = | 172.0 | nM | 6.76 |
| CHEMBL5753503 | — | IC50 | = | 258.0 | nM | 6.59 |
| CHEMBL6046794 | — | IC50 | = | 306.0 | nM | 6.51 |
| CHEMBL6016170 | — | IC50 | = | 416.0 | nM | 6.38 |
| CHEMBL5990752 | — | IC50 | = | 426.0 | nM | 6.37 |
| CHEMBL5976851 | — | IC50 | = | 501.0 | nM | 6.30 |
| CHEMBL6023399 | — | IC50 | = | 531.0 | nM | 6.28 |
| CHEMBL5954666 | — | IC50 | = | 596.0 | nM | 6.22 |
| CHEMBL5827694 | — | IC50 | = | 804.0 | nM | 6.09 |
| CHEMBL5979374 | — | IC50 | = | 824.0 | nM | 6.08 |
| CHEMBL5858089 | — | IC50 | = | 973.0 | nM | 6.01 |
| CHEMBL5991930 | — | IC50 | = | 1026.0 | nM | 5.99 |
| CHEMBL5935125 | — | IC50 | = | 1071.0 | nM | 5.97 |
| CHEMBL5802500 | — | IC50 | = | 1230.0 | nM | 5.91 |
| CHEMBL5820101 | — | IC50 | = | 1327.0 | nM | 5.88 |
| CHEMBL5855478 | — | IC50 | = | 1399.0 | nM | 5.85 |
| CHEMBL6030562 | — | IC50 | = | 1489.0 | nM | 5.83 |
| CHEMBL6040369 | — | IC50 | = | 1850.0 | nM | 5.73 |
| CHEMBL5752694 | — | IC50 | = | 1931.0 | nM | 5.71 |
| CHEMBL5919556 | — | IC50 | = | 1935.0 | nM | 5.71 |
| CHEMBL5841040 | — | IC50 | = | 2053.0 | nM | 5.69 |
| CHEMBL5933911 | — | IC50 | = | 2536.0 | nM | 5.60 |
| CHEMBL5968094 | — | IC50 | = | 2666.0 | nM | 5.57 |
| CHEMBL5823917 | — | IC50 | = | 2806.0 | nM | 5.55 |
| CHEMBL5753333 | — | IC50 | = | 2824.0 | nM | 5.55 |
| CHEMBL5872801 | — | IC50 | = | 2903.0 | nM | 5.54 |
| CHEMBL5873953 | — | IC50 | = | 3027.0 | nM | 5.52 |
| CHEMBL6011006 | — | IC50 | = | 3352.0 | nM | 5.47 |
| CHEMBL5928253 | — | IC50 | = | 3387.0 | nM | 5.47 |
| CHEMBL5888113 | — | IC50 | = | 3724.0 | nM | 5.43 |
| CHEMBL5769990 | — | IC50 | = | 3796.0 | nM | 5.42 |
| CHEMBL5943347 | — | IC50 | = | 3842.0 | nM | 5.42 |
| CHEMBL5864913 | — | IC50 | = | 4611.0 | nM | 5.34 |
| CHEMBL5822857 | — | IC50 | = | 4688.0 | nM | 5.33 |
| CHEMBL5810491 | — | IC50 | = | 4826.0 | nM | 5.32 |
| CHEMBL5750422 | — | IC50 | = | 4817.0 | nM | 5.32 |
| CHEMBL6010123 | — | IC50 | = | 5257.0 | nM | 5.28 |
| CHEMBL5990759 | — | IC50 | = | 5445.0 | nM | 5.26 |
| CHEMBL5840394 | — | IC50 | = | 6360.0 | nM | 5.20 |
| CHEMBL6009171 | — | IC50 | = | 6829.0 | nM | 5.17 |
| CHEMBL5963080 | — | IC50 | = | 7232.0 | nM | 5.14 |
| CHEMBL5981366 | — | IC50 | = | 7258.0 | nM | 5.14 |
| CHEMBL5821652 | — | IC50 | = | 7365.0 | nM | 5.13 |
| CHEMBL5835423 | — | IC50 | = | 7740.0 | nM | 5.11 |
| CHEMBL5967674 | — | IC50 | = | 8173.0 | nM | 5.09 |
| CHEMBL6040301 | — | IC50 | = | 8651.0 | nM | 5.06 |
| CHEMBL5846222 | — | IC50 | = | 9544.0 | nM | 5.02 |
| CHEMBL6041255 | — | IC50 | > | 10000.0 | nM | - |