Assay Detail
Binding
CHEMBL5733190
Review assay metadata, readout intent, target linkage, and publication context from the same page.
Enzymatic Assay: Compounds were tested in an enzymatic assay using human ERK-2 (Mitogen Activated Kinase 1), recombinantly expressed as an n-terminal 6-His fusion protein in E. coli and corresponding to aa 8-360. The substrate used was the fluorescent Omnia peptide S/T17 (Invitrogen of Carlsbad, Calif.; Cat. KNZ1171C). Test compounds were diluted in DMSO in 3-fold serial dilutions at 100× final concentrations. In addition to compound, the assay contained 50 mM HEPES [pH 7.3], 10 mM MgCl2, 2 mM DTT, 0.005% Triton-X100, 5 nM ERK-2 enzyme, 6.25 μM S/T17 peptide substrate and 25 μM ATP (corresponding to the observed Km) for a total reaction volume of 25 μL. The assay was run at ambient temperature in a white 384-well polypropylene plate (Nunc, Inc of Naperville, Ill.; Cat. 267462) collecting data every 50 seconds for approximately 30 minutes on an Envision plate reader (PerkinElmer, Inc. of Waltham, Mass.); Excitation 340 nm/Emission 495 nm.
591
Total Activities
185
Compounds Tested
3
Activity Types
0
Assay Parameters
Assay Information
| Assay Type | Binding |
| Organism | Homo sapiens |
| Confidence | 9 — Direct single protein target |
| Curated By | Autocuration |
Publication
Serine/threonine kinase inhibitors
Activity Statistics
| Type | Count | Avg pChEMBL | Best pChEMBL |
|---|---|---|---|
| IC50 | 197 | 8.82 | 10.70 |
| kon | 197 | - | - |
| k_off | 197 | - | - |
Compounds Tested
| Compound | Name | Phase | Activities | Best pChEMBL |
|---|---|---|---|---|
| CHEMBL5904041 | — | — | 3 | 10.70 |
| CHEMBL6045548 | — | — | 3 | 10.70 |
| CHEMBL5846324 | — | — | 3 | 10.70 |
| CHEMBL5797207 | — | — | 3 | 10.70 |
| CHEMBL5908683 | — | — | 6 | 10.70 |
| CHEMBL5880878 | — | — | 3 | 10.70 |
| CHEMBL5927076 | — | — | 3 | 10.64 |
| CHEMBL5827057 | — | — | 3 | 10.64 |
| CHEMBL5834243 | — | — | 3 | 10.60 |
| CHEMBL5871770 | — | — | 3 | 10.59 |
| CHEMBL5776694 | — | — | 3 | 10.52 |
| CHEMBL5886804 | — | — | 3 | 10.52 |
| CHEMBL5887657 | — | — | 3 | 10.46 |
| CHEMBL5833636 | — | — | 6 | 10.44 |
| CHEMBL5882416 | — | — | 3 | 10.42 |
| CHEMBL5891541 | — | — | 3 | 10.41 |
| CHEMBL5770357 | — | — | 3 | 10.36 |
| CHEMBL5893541 | — | — | 3 | 10.14 |
| CHEMBL5870176 | — | — | 3 | 10.10 |
| CHEMBL6036956 | — | — | 6 | 10.09 |
| CHEMBL5798231 | — | — | 3 | 10.07 |
| CHEMBL6026429 | — | — | 3 | 10.06 |
| CHEMBL5840701 | — | — | 3 | 9.99 |
| CHEMBL5914503 | — | — | 3 | 9.99 |
| CHEMBL5988799 | — | — | 3 | 9.96 |
| CHEMBL5768349 | — | — | 3 | 9.96 |
| CHEMBL6055966 | — | — | 3 | 9.95 |
| CHEMBL5876552 | — | — | 3 | 9.88 |
| CHEMBL5835341 | — | — | 3 | 9.88 |
| CHEMBL5958287 | — | — | 3 | 9.85 |
Activity Data
| Compound | Name | Type | Rel. | Value | Units | pChEMBL |
|---|---|---|---|---|---|---|
| CHEMBL5880878 | — | IC50 | = | 0.02 | nM | 10.70 |
| CHEMBL5846324 | — | IC50 | = | 0.02 | nM | 10.70 |
| CHEMBL5904041 | — | IC50 | = | 0.02 | nM | 10.70 |
| CHEMBL5908683 | — | IC50 | = | 0.02 | nM | 10.70 |
| CHEMBL6045548 | — | IC50 | = | 0.02 | nM | 10.70 |
| CHEMBL5797207 | — | IC50 | = | 0.02 | nM | 10.70 |
| CHEMBL5927076 | — | IC50 | = | 0.023 | nM | 10.64 |
| CHEMBL5827057 | — | IC50 | = | 0.023 | nM | 10.64 |
| CHEMBL5834243 | — | IC50 | = | 0.025 | nM | 10.60 |
| CHEMBL5871770 | — | IC50 | = | 0.026 | nM | 10.59 |
| CHEMBL5776694 | — | IC50 | = | 0.03 | nM | 10.52 |
| CHEMBL5886804 | — | IC50 | = | 0.03 | nM | 10.52 |
| CHEMBL5887657 | — | IC50 | = | 0.035 | nM | 10.46 |
| CHEMBL5833636 | — | IC50 | = | 0.036 | nM | 10.44 |
| CHEMBL5882416 | — | IC50 | = | 0.038 | nM | 10.42 |
| CHEMBL5891541 | — | IC50 | = | 0.039 | nM | 10.41 |
| CHEMBL5770357 | — | IC50 | = | 0.044 | nM | 10.36 |
| CHEMBL5893541 | — | IC50 | = | 0.073 | nM | 10.14 |
| CHEMBL5870176 | — | IC50 | = | 0.079 | nM | 10.10 |
| CHEMBL6036956 | — | IC50 | = | 0.082 | nM | 10.09 |
| CHEMBL5798231 | — | IC50 | = | 0.085 | nM | 10.07 |
| CHEMBL6026429 | — | IC50 | = | 0.088 | nM | 10.06 |
| CHEMBL5840701 | — | IC50 | = | 0.102 | nM | 9.99 |
| CHEMBL5914503 | — | IC50 | = | 0.103 | nM | 9.99 |
| CHEMBL5988799 | — | IC50 | = | 0.109 | nM | 9.96 |
| CHEMBL5768349 | — | IC50 | = | 0.111 | nM | 9.96 |
| CHEMBL6055966 | — | IC50 | = | 0.113 | nM | 9.95 |
| CHEMBL5876552 | — | IC50 | = | 0.131 | nM | 9.88 |
| CHEMBL5835341 | — | IC50 | = | 0.132 | nM | 9.88 |
| CHEMBL5958287 | — | IC50 | = | 0.14 | nM | 9.85 |
| CHEMBL5970498 | — | IC50 | = | 0.149 | nM | 9.83 |
| CHEMBL5843088 | — | IC50 | = | 0.149 | nM | 9.83 |
| CHEMBL5959724 | — | IC50 | = | 0.168 | nM | 9.78 |
| CHEMBL5958147 | — | IC50 | = | 0.173 | nM | 9.76 |
| CHEMBL6024181 | — | IC50 | = | 0.215 | nM | 9.67 |
| CHEMBL5824130 | — | IC50 | = | 0.273 | nM | 9.56 |
| CHEMBL6054742 | — | IC50 | = | 0.311 | nM | 9.51 |
| CHEMBL5959280 | — | IC50 | = | 0.311 | nM | 9.51 |
| CHEMBL5821708 | — | IC50 | = | 0.311 | nM | 9.51 |
| CHEMBL5750619 | — | IC50 | = | 0.316 | nM | 9.50 |
| CHEMBL5783483 | — | IC50 | = | 0.316 | nM | 9.50 |
| CHEMBL5762132 | — | IC50 | = | 0.38 | nM | 9.42 |
| CHEMBL5988077 | — | IC50 | = | 0.39 | nM | 9.41 |
| CHEMBL5988990 | — | IC50 | = | 0.408 | nM | 9.39 |
| CHEMBL5776279 | — | IC50 | = | 0.47 | nM | 9.33 |
| CHEMBL5848245 | — | IC50 | = | 0.465 | nM | 9.33 |
| CHEMBL6061750 | — | IC50 | = | 0.512 | nM | 9.29 |
| CHEMBL5821824 | — | IC50 | = | 0.514 | nM | 9.29 |
| CHEMBL5984955 | — | IC50 | = | 0.6 | nM | 9.22 |
| CHEMBL5928095 | — | IC50 | = | 0.6 | nM | 9.22 |