Assay Detail
Binding
CHEMBL5734665
Review assay metadata, readout intent, target linkage, and publication context from the same page.
In Vitro Kinase Assay: Compound of the invention were screened in an in vitro kinase assay against several members of the TGFβ family of Ser/Thr kinases. The kinases tested were ALK1 (ACVRL1), ALK2 (ACVR1), ALK3 (BMPR1A), ALK4 (ACVR1B), ALK5 (TGFBR1), and ALK6 (BMPR1B). Standard kinase testing conditions and techniques were employed. For each case, specific kinase/substrate pairs along with required cofactors were prepared in reaction buffer. Compound of the invention were delivered into the reaction, followed 15-20 min later by addition of a mixture of ATP and 33P ATP to a final concentration of 10 μM. Reactions were carried out at RT for 120 min, followed by spotting of the reactions onto P81 ion exchange filter paper. Unbound phosphate was removed by extensive washing of filters in 0.75% phosphoric acid. Kinase activity data was expressed as the percent of remaining kinase activity in test samples compared to vehicle.
108
Total Activities
36
Compounds Tested
3
Activity Types
0
Assay Parameters
Assay Information
| Assay Type | Binding |
| Confidence | 8 — Homologous single protein target |
| Curated By | Autocuration |
Publication
Compounds and methods of use
Activity Statistics
| Type | Count | Avg pChEMBL | Best pChEMBL |
|---|---|---|---|
| IC50 | 36 | 5.26 | 5.82 |
| kon | 36 | - | - |
| k_off | 36 | - | - |
Compounds Tested
| Compound | Name | Phase | Activities | Best pChEMBL |
|---|---|---|---|---|
| CHEMBL5768318 | — | — | 3 | 5.82 |
| CHEMBL5813038 | — | — | 3 | 5.71 |
| CHEMBL6036046 | — | — | 3 | 5.67 |
| CHEMBL5897331 | — | — | 3 | 5.59 |
| CHEMBL5768693 | — | — | 3 | 5.46 |
| CHEMBL5903130 | — | — | 3 | 5.43 |
| CHEMBL5290769 | — | — | 3 | 5.34 |
| CHEMBL5761663 | — | — | 3 | 5.33 |
| CHEMBL5288333 | — | — | 3 | 5.30 |
| CHEMBL5995233 | — | — | 3 | 5.28 |
| CHEMBL5749427 | — | — | 3 | 5.25 |
| CHEMBL5800993 | — | — | 3 | 5.23 |
| CHEMBL5285430 | — | — | 3 | 5.23 |
| CHEMBL5983777 | — | — | 3 | 5.18 |
| CHEMBL5857202 | — | — | 3 | 5.17 |
| CHEMBL5291016 | — | — | 3 | 5.16 |
| CHEMBL5095192 | PF-06952229 | 1.0 | 3 | 5.14 |
| CHEMBL5964579 | — | — | 3 | 5.14 |
| CHEMBL5818096 | — | — | 3 | 5.09 |
| CHEMBL5957596 | — | — | 3 | 5.08 |
| CHEMBL6025407 | — | — | 3 | 5.04 |
| CHEMBL5289691 | — | — | 3 | 5.00 |
| CHEMBL5972400 | — | — | 3 | 4.88 |
| CHEMBL5975807 | — | — | 3 | 4.78 |
| CHEMBL5788695 | — | — | 3 | - |
| CHEMBL5834461 | — | — | 3 | - |
| CHEMBL5889454 | — | — | 3 | - |
| CHEMBL5818729 | — | — | 3 | - |
| CHEMBL5934556 | — | — | 3 | - |
| CHEMBL5926913 | — | — | 3 | - |
Activity Data
| Compound | Name | Type | Rel. | Value | Units | pChEMBL |
|---|---|---|---|---|---|---|
| CHEMBL5768318 | — | IC50 | = | 1530.0 | nM | 5.82 |
| CHEMBL5813038 | — | IC50 | = | 1940.0 | nM | 5.71 |
| CHEMBL6036046 | — | IC50 | = | 2130.0 | nM | 5.67 |
| CHEMBL5897331 | — | IC50 | = | 2540.0 | nM | 5.59 |
| CHEMBL5768693 | — | IC50 | = | 3440.0 | nM | 5.46 |
| CHEMBL5903130 | — | IC50 | = | 3700.0 | nM | 5.43 |
| CHEMBL5290769 | — | IC50 | = | 4590.0 | nM | 5.34 |
| CHEMBL5761663 | — | IC50 | = | 4670.0 | nM | 5.33 |
| CHEMBL5288333 | — | IC50 | = | 5010.0 | nM | 5.30 |
| CHEMBL5995233 | — | IC50 | = | 5200.0 | nM | 5.28 |
| CHEMBL5749427 | — | IC50 | = | 5670.0 | nM | 5.25 |
| CHEMBL5800993 | — | IC50 | = | 5900.0 | nM | 5.23 |
| CHEMBL5285430 | — | IC50 | = | 5850.0 | nM | 5.23 |
| CHEMBL5983777 | — | IC50 | = | 6570.0 | nM | 5.18 |
| CHEMBL5857202 | — | IC50 | = | 6750.0 | nM | 5.17 |
| CHEMBL5291016 | — | IC50 | = | 6920.0 | nM | 5.16 |
| CHEMBL5095192 | PF-06952229 | IC50 | = | 7290.0 | nM | 5.14 |
| CHEMBL5964579 | — | IC50 | = | 7300.0 | nM | 5.14 |
| CHEMBL5818096 | — | IC50 | = | 8200.0 | nM | 5.09 |
| CHEMBL5957596 | — | IC50 | = | 8410.0 | nM | 5.08 |
| CHEMBL6025407 | — | IC50 | = | 9120.0 | nM | 5.04 |
| CHEMBL5289691 | — | IC50 | = | 10000.0 | nM | 5.00 |
| CHEMBL5972400 | — | IC50 | = | 13100.0 | nM | 4.88 |
| CHEMBL5975807 | — | IC50 | = | 16500.0 | nM | 4.78 |
| CHEMBL5995233 | — | k_off | = | - | s-1 | - |
| CHEMBL5972400 | — | kon | = | - | — | - |
| CHEMBL5788695 | — | kon | = | - | — | - |
| CHEMBL5897331 | — | k_off | = | - | s-1 | - |
| CHEMBL5834461 | — | IC50 | > | 10000.0 | nM | - |
| CHEMBL5834461 | — | kon | = | - | — | - |
| CHEMBL5834461 | — | k_off | = | - | s-1 | - |
| CHEMBL5788695 | — | IC50 | > | 10000.0 | nM | - |
| CHEMBL5813038 | — | k_off | = | - | s-1 | - |
| CHEMBL5903130 | — | k_off | = | - | s-1 | - |
| CHEMBL5903130 | — | kon | = | - | — | - |
| CHEMBL5897331 | — | kon | = | - | — | - |
| CHEMBL5813038 | — | kon | = | - | — | - |
| CHEMBL5995233 | — | kon | = | - | — | - |
| CHEMBL5926913 | — | k_off | = | - | s-1 | - |
| CHEMBL5926913 | — | kon | = | - | — | - |
| CHEMBL5926913 | — | IC50 | > | 100000.0 | nM | - |
| CHEMBL5850928 | — | k_off | = | - | s-1 | - |
| CHEMBL5850928 | — | kon | = | - | — | - |
| CHEMBL5850928 | — | IC50 | > | 100000.0 | nM | - |
| CHEMBL6006931 | — | k_off | = | - | s-1 | - |
| CHEMBL6006931 | — | kon | = | - | — | - |
| CHEMBL6006931 | — | IC50 | > | 100000.0 | nM | - |
| CHEMBL5749427 | — | k_off | = | - | s-1 | - |
| CHEMBL5749427 | — | kon | = | - | — | - |
| CHEMBL5818096 | — | kon | = | - | — | - |