Assay Detail
Binding
CHEMBL5734775
Review assay metadata, readout intent, target linkage, and publication context from the same page.
luminescent kinase assay: Inhibition of TgCDPK1 and CpCDPK1 was determined using a luminescent kinase assay which measures ATP depletion in the presence of the Syntide 2 peptide substrate (KinaseGlo). (U.S. Provisional Patent Application No. 61/299,286, and reference 9) Similar to TgCDPK1, exogenous calcium was necessary for CpCDPK1 to possess maximum catalytic activity (data not shown). Notably, both kinases were tested at the same ATP concentration which allows direct comparison of inhibitor potencies due to these enzymes possessing similar Kms for this cofactor. (20)Encouraged by the similar potency of inhibitor 3 against TgCDPK1 (IC50=150±20 nM) and CpCDPK1 (IC50=130±40 nM), pyrazolopyrimidine analogues that contain a naphthylmethylene group at the 3-position and various alkyl substituents at the 1-position were tested for their ability to inhibit both kinases.
225
Total Activities
75
Compounds Tested
3
Activity Types
0
Assay Parameters
Assay Information
| Assay Type | Binding |
| Organism | Homo sapiens |
| Confidence | 9 — Direct single protein target |
| Curated By | Autocuration |
Publication
Compositions and methods for treating toxoplasmosis, cryptosporidiosis, and other apicomplexan protozoan related diseases
Activity Statistics
| Type | Count | Avg pChEMBL | Best pChEMBL |
|---|---|---|---|
| IC50 | 75 | 6.03 | 8.00 |
| kon | 75 | - | - |
| k_off | 75 | - | - |
Compounds Tested
| Compound | Name | Phase | Activities | Best pChEMBL |
|---|---|---|---|---|
| CHEMBL2059165 | — | — | 3 | 8.00 |
| CHEMBL5784931 | — | — | 3 | 7.48 |
| CHEMBL1785012 | — | — | 3 | 7.16 |
| CHEMBL1241489 | — | — | 3 | 7.12 |
| CHEMBL5746276 | — | — | 3 | 7.06 |
| CHEMBL1784972 | — | — | 3 | 7.05 |
| CHEMBL170250 | — | — | 3 | 7.03 |
| CHEMBL406845 | — | — | 3 | 6.76 |
| CHEMBL5860627 | — | — | 3 | 6.71 |
| CHEMBL1785014 | — | — | 3 | 6.70 |
| CHEMBL1242384 | — | — | 3 | 6.62 |
| CHEMBL2030556 | — | — | 3 | 6.52 |
| CHEMBL2078865 | — | — | 3 | 6.50 |
| CHEMBL2079136 | — | — | 3 | 6.45 |
| CHEMBL1784969 | — | — | 3 | 6.44 |
| CHEMBL5774425 | — | — | 3 | 6.39 |
| CHEMBL2079317 | — | — | 3 | 6.30 |
| CHEMBL2059166 | — | — | 3 | 6.26 |
| CHEMBL2078794 | — | — | 3 | 6.20 |
| CHEMBL5896441 | — | — | 3 | 6.19 |
| CHEMBL2079167 | — | — | 3 | 6.17 |
| CHEMBL1785020 | — | — | 3 | 6.09 |
| CHEMBL2079214 | — | — | 3 | 6.08 |
| CHEMBL5932426 | — | — | 3 | 6.01 |
| CHEMBL5918917 | — | — | 3 | 5.97 |
| CHEMBL2079215 | — | — | 3 | 5.94 |
| CHEMBL5767089 | — | — | 3 | 5.91 |
| CHEMBL5875647 | — | — | 3 | 5.90 |
| CHEMBL2079137 | — | — | 3 | 5.80 |
| CHEMBL2078815 | — | — | 3 | 5.79 |
Activity Data
| Compound | Name | Type | Rel. | Value | Units | pChEMBL |
|---|---|---|---|---|---|---|
| CHEMBL2059165 | — | IC50 | = | 10.0 | nM | 8.00 |
| CHEMBL5784931 | — | IC50 | = | 33.3 | nM | 7.48 |
| CHEMBL1785012 | — | IC50 | = | 70.0 | nM | 7.16 |
| CHEMBL1241489 | — | IC50 | = | 75.0 | nM | 7.12 |
| CHEMBL5746276 | — | IC50 | = | 86.2 | nM | 7.06 |
| CHEMBL1784972 | — | IC50 | = | 90.0 | nM | 7.05 |
| CHEMBL170250 | — | IC50 | = | 94.0 | nM | 7.03 |
| CHEMBL406845 | — | IC50 | = | 172.0 | nM | 6.76 |
| CHEMBL5860627 | — | IC50 | = | 194.0 | nM | 6.71 |
| CHEMBL1785014 | — | IC50 | = | 200.0 | nM | 6.70 |
| CHEMBL1242384 | — | IC50 | = | 238.0 | nM | 6.62 |
| CHEMBL2030556 | — | IC50 | = | 304.0 | nM | 6.52 |
| CHEMBL2078865 | — | IC50 | = | 316.0 | nM | 6.50 |
| CHEMBL2079136 | — | IC50 | = | 353.0 | nM | 6.45 |
| CHEMBL1784969 | — | IC50 | = | 360.0 | nM | 6.44 |
| CHEMBL5774425 | — | IC50 | = | 403.0 | nM | 6.39 |
| CHEMBL2079317 | — | IC50 | = | 497.0 | nM | 6.30 |
| CHEMBL2059166 | — | IC50 | = | 554.0 | nM | 6.26 |
| CHEMBL2078794 | — | IC50 | = | 636.0 | nM | 6.20 |
| CHEMBL5896441 | — | IC50 | = | 649.0 | nM | 6.19 |
| CHEMBL2079167 | — | IC50 | = | 675.0 | nM | 6.17 |
| CHEMBL1785020 | — | IC50 | = | 820.0 | nM | 6.09 |
| CHEMBL2079214 | — | IC50 | = | 838.0 | nM | 6.08 |
| CHEMBL5932426 | — | IC50 | = | 969.0 | nM | 6.01 |
| CHEMBL5918917 | — | IC50 | = | 1073.0 | nM | 5.97 |
| CHEMBL2079215 | — | IC50 | = | 1153.0 | nM | 5.94 |
| CHEMBL5767089 | — | IC50 | = | 1230.0 | nM | 5.91 |
| CHEMBL5875647 | — | IC50 | = | 1267.0 | nM | 5.90 |
| CHEMBL2079137 | — | IC50 | = | 1589.0 | nM | 5.80 |
| CHEMBL2078815 | — | IC50 | = | 1636.0 | nM | 5.79 |
| CHEMBL2079232 | — | IC50 | = | 1680.0 | nM | 5.78 |
| CHEMBL5983391 | — | IC50 | = | 1701.0 | nM | 5.77 |
| CHEMBL1242754 | — | IC50 | = | 1700.0 | nM | 5.77 |
| CHEMBL1785021 | — | IC50 | = | 1700.0 | nM | 5.77 |
| CHEMBL5776934 | — | IC50 | = | 1798.0 | nM | 5.75 |
| CHEMBL5889864 | — | IC50 | = | 2010.0 | nM | 5.70 |
| CHEMBL2079138 | — | IC50 | = | 2360.0 | nM | 5.63 |
| CHEMBL2079213 | — | IC50 | = | 2943.0 | nM | 5.53 |
| CHEMBL5861083 | — | IC50 | = | 4159.0 | nM | 5.38 |
| CHEMBL5814019 | — | IC50 | = | 4790.0 | nM | 5.32 |
| CHEMBL2079229 | — | IC50 | = | 4861.0 | nM | 5.31 |
| CHEMBL2030558 | — | IC50 | = | 5982.0 | nM | 5.22 |
| CHEMBL1231371 | — | IC50 | = | 6000.0 | nM | 5.22 |
| CHEMBL5909447 | — | IC50 | = | 6350.0 | nM | 5.20 |
| CHEMBL2078805 | — | IC50 | = | 7340.0 | nM | 5.13 |
| CHEMBL6047364 | — | IC50 | = | 7326.0 | nM | 5.13 |
| CHEMBL5785484 | — | IC50 | = | 7845.0 | nM | 5.11 |
| CHEMBL573578 | — | IC50 | = | 7700.0 | nM | 5.11 |
| CHEMBL1231370 | — | IC50 | = | 7900.0 | nM | 5.10 |
| CHEMBL2078771 | — | IC50 | = | 8040.0 | nM | 5.09 |