Assay Detail
Binding
CHEMBL5734810
Review assay metadata, readout intent, target linkage, and publication context from the same page.
Assay of p38 Kinase Activity: Experimental Method: 40 nM p38a was added into a reaction buffer, which include 20 mM HEPES (4-hydroxyethylpiperazine ethanesulfonic acid), 5 mM MgCl2 and 1 mM DTT (dithiothreitol) at pH 7.4. 40 μM phosphorylated peptide substrate was added, followed by different concentrations of drugs and 100 μM ATP (adenosine triphosphate), and reacted for 90 minutes in dark. The fluorescence values were determined at excitation and emission wavelengths of 544 nM and 590 nM respectively. The IC50 values of the compounds were obtained according to the fluorescence values.
72
Total Activities
24
Compounds Tested
3
Activity Types
0
Assay Parameters
Assay Information
| Assay Type | Binding |
| Organism | Homo sapiens |
| Confidence | 9 — Direct single protein target |
| Curated By | Autocuration |
Publication
Benzamide derivative
Activity Statistics
| Type | Count | Avg pChEMBL | Best pChEMBL |
|---|---|---|---|
| IC50 | 24 | 6.14 | 6.26 |
| kon | 24 | - | - |
| k_off | 24 | - | - |
Compounds Tested
| Compound | Name | Phase | Activities | Best pChEMBL |
|---|---|---|---|---|
| CHEMBL5845520 | — | — | 3 | 6.26 |
| CHEMBL5849787 | — | — | 3 | 6.26 |
| CHEMBL5851332 | — | — | 3 | 6.26 |
| CHEMBL6018153 | — | — | 3 | 6.26 |
| CHEMBL5954596 | — | — | 3 | 6.26 |
| CHEMBL6053768 | — | — | 3 | 6.26 |
| CHEMBL5888672 | — | — | 3 | 6.26 |
| CHEMBL1088752 | LOSMAPIMOD | 3.0 | 3 | 5.26 |
| CHEMBL5959448 | — | — | 3 | - |
| CHEMBL5977503 | — | — | 3 | - |
| CHEMBL6021110 | — | — | 3 | - |
| CHEMBL5838074 | — | — | 3 | - |
| CHEMBL6000030 | — | — | 3 | - |
| CHEMBL5742619 | — | — | 3 | - |
| CHEMBL5864316 | — | — | 3 | - |
| CHEMBL5905513 | — | — | 3 | - |
| CHEMBL5875721 | — | — | 3 | - |
| CHEMBL5753624 | — | — | 3 | - |
| CHEMBL5972656 | — | — | 3 | - |
| CHEMBL5825952 | — | — | 3 | - |
| CHEMBL6032738 | — | — | 3 | - |
| CHEMBL6001751 | — | — | 3 | - |
| CHEMBL5959348 | — | — | 3 | - |
| CHEMBL5948708 | — | — | 3 | - |
Activity Data
| Compound | Name | Type | Rel. | Value | Units | pChEMBL |
|---|---|---|---|---|---|---|
| CHEMBL5851332 | — | IC50 | = | 550.0 | nM | 6.26 |
| CHEMBL5888672 | — | IC50 | = | 550.0 | nM | 6.26 |
| CHEMBL6018153 | — | IC50 | = | 550.0 | nM | 6.26 |
| CHEMBL5954596 | — | IC50 | = | 550.0 | nM | 6.26 |
| CHEMBL5849787 | — | IC50 | = | 550.0 | nM | 6.26 |
| CHEMBL6053768 | — | IC50 | = | 550.0 | nM | 6.26 |
| CHEMBL5845520 | — | IC50 | = | 550.0 | nM | 6.26 |
| CHEMBL1088752 | LOSMAPIMOD | IC50 | = | 5500.0 | nM | 5.26 |
| CHEMBL5742619 | — | IC50 | < | 100.0 | nM | - |
| CHEMBL6021110 | — | IC50 | < | 100.0 | nM | - |
| CHEMBL1088752 | LOSMAPIMOD | k_off | = | - | s-1 | - |
| CHEMBL5742619 | — | k_off | = | - | s-1 | - |
| CHEMBL6000030 | — | IC50 | < | 100.0 | nM | - |
| CHEMBL6000030 | — | kon | = | - | — | - |
| CHEMBL6000030 | — | k_off | = | - | s-1 | - |
| CHEMBL5838074 | — | IC50 | < | 100.0 | nM | - |
| CHEMBL5838074 | — | kon | = | - | — | - |
| CHEMBL5864316 | — | k_off | = | - | s-1 | - |
| CHEMBL5972656 | — | kon | = | - | — | - |
| CHEMBL5972656 | — | k_off | = | - | s-1 | - |
| CHEMBL6032738 | — | k_off | = | - | s-1 | - |
| CHEMBL6032738 | — | kon | = | - | — | - |
| CHEMBL6032738 | — | IC50 | < | 100.0 | nM | - |
| CHEMBL5825952 | — | k_off | = | - | s-1 | - |
| CHEMBL5825952 | — | kon | = | - | — | - |
| CHEMBL5825952 | — | IC50 | < | 100.0 | nM | - |
| CHEMBL5849787 | — | k_off | = | - | s-1 | - |
| CHEMBL5849787 | — | kon | = | - | — | - |
| CHEMBL5954596 | — | kon | = | - | — | - |
| CHEMBL5888672 | — | kon | = | - | — | - |
| CHEMBL5864316 | — | kon | = | - | — | - |
| CHEMBL5742619 | — | kon | = | - | — | - |
| CHEMBL5864316 | — | IC50 | < | 100.0 | nM | - |
| CHEMBL5905513 | — | k_off | = | - | s-1 | - |
| CHEMBL5905513 | — | kon | = | - | — | - |
| CHEMBL5905513 | — | IC50 | < | 100.0 | nM | - |
| CHEMBL5875721 | — | k_off | = | - | s-1 | - |
| CHEMBL5875721 | — | kon | = | - | — | - |
| CHEMBL5875721 | — | IC50 | < | 100.0 | nM | - |
| CHEMBL5888672 | — | k_off | = | - | s-1 | - |
| CHEMBL6021110 | — | kon | = | - | — | - |
| CHEMBL5845520 | — | k_off | = | - | s-1 | - |
| CHEMBL5845520 | — | kon | = | - | — | - |
| CHEMBL5838074 | — | k_off | = | - | s-1 | - |
| CHEMBL5959448 | — | IC50 | < | 100.0 | nM | - |
| CHEMBL1088752 | LOSMAPIMOD | kon | = | - | — | - |
| CHEMBL5977503 | — | k_off | = | - | s-1 | - |
| CHEMBL5977503 | — | kon | = | - | — | - |
| CHEMBL5977503 | — | IC50 | < | 100.0 | nM | - |
| CHEMBL6021110 | — | k_off | = | - | s-1 | - |