Skip to main content
Free research Free research Free assay review with research home and workspace preview
Quick search ChEMBL 36
Assay Detail

CHEMBL5734977

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Enzymatic Activity Assay: DGAT2 activity was determined by measuring the amount of enzymatic product triolein (1,2,3-Tri(cis-9-octadecenoyl)glycerol) using the membrane prep mentioned above. The assay was carried out in deep well 384 plates in a final volume of 40 μL at room temperature. The assay mixture contained the following: assay buffer (100 mM Tris.Cl, pH 7.0, 20 mM MgCl2, 5% ethanol), 25 μM of diolein, 10 μM of oleoyl-CoA and 10 ng/μL of DGAT2 membrane.
423
Total Activities
138
Compounds Tested
3
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Diacylglycerol O-acyltransferase 2 (CHEMBL5853)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Tetrahydroisoquinoline derivatives useful as inhibitors of diacylglyceride O-acyltransferase 2
(2020)

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 141 6.49 9.18
kon 141 - -
k_off 141 - -

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL6000290 3 9.18
CHEMBL5991270 3 8.95
CHEMBL6060795 3 8.66
CHEMBL5849208 3 8.52
CHEMBL5938206 3 8.44
CHEMBL5775051 3 8.44
CHEMBL5942569 3 8.24
CHEMBL5938338 3 8.24
CHEMBL5753425 3 8.21
CHEMBL5860266 3 8.08
CHEMBL5882372 3 8.07
CHEMBL6040965 3 8.03
CHEMBL5985921 3 8.01
CHEMBL5755785 3 8.00
CHEMBL5954660 3 7.98
CHEMBL5802239 3 7.94
CHEMBL6058144 3 7.94
CHEMBL5965391 3 7.94
CHEMBL6013100 3 7.93
CHEMBL5891481 3 7.87
CHEMBL5910959 3 7.87
CHEMBL5802038 3 7.86
CHEMBL6041971 6 7.81
CHEMBL5908278 3 7.79
CHEMBL6011379 3 7.71
CHEMBL5805848 3 7.70
CHEMBL6052343 3 7.69
CHEMBL5770881 3 7.65
CHEMBL5841950 3 7.62
CHEMBL6063237 3 7.62

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL6000290 IC50 = 0.66 nM 9.18
CHEMBL5991270 IC50 = 1.13 nM 8.95
CHEMBL6060795 IC50 = 2.2 nM 8.66
CHEMBL5849208 IC50 = 3.0 nM 8.52
CHEMBL5938206 IC50 = 3.6 nM 8.44
CHEMBL5775051 IC50 = 3.6 nM 8.44
CHEMBL5942569 IC50 = 5.8 nM 8.24
CHEMBL5938338 IC50 = 5.78 nM 8.24
CHEMBL5753425 IC50 = 6.1 nM 8.21
CHEMBL5860266 IC50 = 8.3 nM 8.08
CHEMBL5882372 IC50 = 8.5 nM 8.07
CHEMBL6040965 IC50 = 9.3 nM 8.03
CHEMBL5985921 IC50 = 9.88 nM 8.01
CHEMBL5755785 IC50 = 10.0 nM 8.00
CHEMBL5954660 IC50 = 10.4 nM 7.98
CHEMBL6058144 IC50 = 11.5 nM 7.94
CHEMBL5802239 IC50 = 11.4 nM 7.94
CHEMBL5965391 IC50 = 11.6 nM 7.94
CHEMBL6013100 IC50 = 11.7 nM 7.93
CHEMBL5910959 IC50 = 13.6 nM 7.87
CHEMBL5891481 IC50 = 13.4 nM 7.87
CHEMBL5802038 IC50 = 13.8 nM 7.86
CHEMBL6041971 IC50 = 15.5 nM 7.81
CHEMBL6041971 IC50 = 15.5 nM 7.81
CHEMBL5908278 IC50 = 16.1 nM 7.79
CHEMBL6011379 IC50 = 19.6 nM 7.71
CHEMBL5805848 IC50 = 20.2 nM 7.70
CHEMBL6052343 IC50 = 20.4 nM 7.69
CHEMBL5770881 IC50 = 22.6 nM 7.65
CHEMBL5841950 IC50 = 24.0 nM 7.62
CHEMBL6063237 IC50 = 23.8 nM 7.62
CHEMBL6001413 IC50 = 31.0 nM 7.51
CHEMBL5773264 IC50 = 34.0 nM 7.47
CHEMBL5841307 IC50 = 52.0 nM 7.28
CHEMBL5745828 IC50 = 59.8 nM 7.22
CHEMBL5913214 IC50 = 82.3 nM 7.08
CHEMBL5751746 IC50 = 87.0 nM 7.06
CHEMBL5942728 IC50 = 89.8 nM 7.05
CHEMBL6011523 IC50 = 95.6 nM 7.02
CHEMBL5919685 IC50 = 98.0 nM 7.01
CHEMBL5837225 IC50 = 97.6 nM 7.01
CHEMBL5915079 IC50 = 100.0 nM 7.00
CHEMBL6043343 IC50 = 112.0 nM 6.95
CHEMBL5815118 IC50 = 126.0 nM 6.90
CHEMBL5891025 IC50 = 144.0 nM 6.84
CHEMBL6050219 IC50 = 155.0 nM 6.81
CHEMBL5891891 IC50 = 166.0 nM 6.78
CHEMBL5811365 IC50 = 173.0 nM 6.76
CHEMBL5884430 IC50 = 176.0 nM 6.75
CHEMBL6046359 IC50 = 209.0 nM 6.68