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Assay Detail

CHEMBL5736328

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Binding
Measurement of Inhibitory Effect on FGFR3 Kinase Activity: The inhibitory effect of the compounds on FGFR3 kinase activity was measured according to the method of Test Example 3. Purified recombinant human FGFR3 protein was purchased from Carna Biosciences, Inc. The final concentration of ATP was 50 μM.
21
Total Activities
6
Compounds Tested
3
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Fibroblast growth factor receptor 3 (CHEMBL2742)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Antitumor drug for intermittent administration of FGFR inhibitor
(2021)

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 7 9.45 10.00
kon 7 - -
k_off 7 - -

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL3701241 6 10.00
CHEMBL3701239 3 9.70
CHEMBL3701238 FUTIBATINIB 4.0 3 9.30
CHEMBL3701262 3 9.30
CHEMBL3701267 3 9.15
CHEMBL3701275 3 9.15

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL3701241 IC50 = 0.1 nM 10.00
CHEMBL3701239 IC50 = 0.2 nM 9.70
CHEMBL3701241 IC50 = 0.3 nM 9.52
CHEMBL3701262 IC50 = 0.5 nM 9.30
CHEMBL3701238 FUTIBATINIB IC50 = 0.5 nM 9.30
CHEMBL3701275 IC50 = 0.7 nM 9.15
CHEMBL3701267 IC50 = 0.7 nM 9.15
CHEMBL3701238 FUTIBATINIB kon = - -
CHEMBL3701238 FUTIBATINIB k_off = - s-1 -
CHEMBL3701239 kon = - -
CHEMBL3701239 k_off = - s-1 -
CHEMBL3701241 kon = - -
CHEMBL3701241 k_off = - s-1 -
CHEMBL3701262 k_off = - s-1 -
CHEMBL3701241 kon = - -
CHEMBL3701241 k_off = - s-1 -
CHEMBL3701267 kon = - -
CHEMBL3701267 k_off = - s-1 -
CHEMBL3701275 kon = - -
CHEMBL3701275 k_off = - s-1 -
CHEMBL3701262 kon = - -