Assay Detail
Binding
CHEMBL5736985
Review assay metadata, readout intent, target linkage, and publication context from the same page.
Enzymatic Assay: HDAC5 reagents: N-terminal GST tagged HDAC5 was purchased from BPS Bioscience [catalog #50045]. Assays were performed with buffer containing 20 mM HEPES, pH 8.0 [Boston BioProducts, catalog #BB-104, 1M stock], 137 mM NaCl [Sigma, catalog #S5150, 5M stock], 2.7 mM KCl [BioChemika, catalog #87526, 4M stock], 1 mM MgCl2 [Fluka, catalog #63020, 1M stock], and 0.05% BSA (Fraction V) [Invitrogen, catalog #15260, 7.5% stock]. The HDAC5 enzyme was run at the final concentration of 0.447 nM. Boc-Lys(TFA)-AMC substrate [Bachem, catalog #1-1985.0050], used to evaluate enzyme activity, was added at the final concentration of 60 uM. To enable detection of the signal, Developer II [BioMol Research Laboratories, catalog #KI-176] was added at a 1:200 dilution to the stop solution, which also included 20 uM trichostatin A (TSA) [Sigma, catalog #T8552] to ensure complete termination of the reaction.
540
Total Activities
179
Compounds Tested
3
Activity Types
0
Assay Parameters
Assay Information
| Assay Type | Binding |
| Organism | Homo sapiens |
| Confidence | 9 — Direct single protein target |
| Curated By | Autocuration |
Publication
3-aryl- heteroaryl substituted 5-trifluoromethyl oxadiazoles as histonedeacetylase 6 (HDAC6) inhibitors
Activity Statistics
| Type | Count | Avg pChEMBL | Best pChEMBL |
|---|---|---|---|
| IC50 | 180 | 6.72 | 8.70 |
| kon | 180 | - | - |
| k_off | 180 | - | - |
Compounds Tested
| Compound | Name | Phase | Activities | Best pChEMBL |
|---|---|---|---|---|
| CHEMBL5964075 | — | — | 3 | 8.70 |
| CHEMBL5891273 | — | — | 3 | 8.52 |
| CHEMBL5884740 | — | — | 3 | 8.30 |
| CHEMBL5879893 | — | — | 3 | 8.15 |
| CHEMBL5751100 | — | — | 3 | 8.15 |
| CHEMBL5838626 | — | — | 3 | 8.08 |
| CHEMBL5937939 | — | — | 3 | 8.02 |
| CHEMBL5898733 | — | — | 3 | 7.96 |
| CHEMBL5981827 | — | — | 3 | 7.77 |
| CHEMBL5763295 | — | — | 3 | 7.75 |
| CHEMBL5850094 | — | — | 3 | 7.75 |
| CHEMBL6024336 | — | — | 3 | 7.72 |
| CHEMBL5838830 | — | — | 3 | 7.72 |
| CHEMBL5746748 | — | — | 3 | 7.68 |
| CHEMBL5864393 | — | — | 3 | 7.68 |
| CHEMBL5954175 | — | — | 3 | 7.66 |
| CHEMBL5747111 | — | — | 3 | 7.62 |
| CHEMBL5998021 | — | — | 3 | 7.60 |
| CHEMBL5840014 | — | — | 3 | 7.58 |
| CHEMBL5809280 | — | — | 3 | 7.57 |
| CHEMBL5948563 | — | — | 3 | 7.55 |
| CHEMBL6009283 | — | — | 3 | 7.52 |
| CHEMBL5892520 | — | — | 3 | 7.51 |
| CHEMBL5954639 | — | — | 3 | 7.51 |
| CHEMBL6038749 | — | — | 3 | 7.51 |
| CHEMBL5751647 | — | — | 3 | 7.47 |
| CHEMBL5913853 | — | — | 3 | 7.44 |
| CHEMBL5787808 | — | — | 3 | 7.43 |
| CHEMBL5940065 | — | — | 3 | 7.38 |
| CHEMBL5995237 | — | — | 3 | 7.32 |
Activity Data
| Compound | Name | Type | Rel. | Value | Units | pChEMBL |
|---|---|---|---|---|---|---|
| CHEMBL5964075 | — | IC50 | = | 2.0 | nM | 8.70 |
| CHEMBL5891273 | — | IC50 | = | 3.0 | nM | 8.52 |
| CHEMBL5884740 | — | IC50 | = | 5.0 | nM | 8.30 |
| CHEMBL5879893 | — | IC50 | = | 7.0 | nM | 8.15 |
| CHEMBL5751100 | — | IC50 | = | 7.0 | nM | 8.15 |
| CHEMBL5838626 | — | IC50 | = | 8.4 | nM | 8.08 |
| CHEMBL5937939 | — | IC50 | = | 9.6 | nM | 8.02 |
| CHEMBL5898733 | — | IC50 | = | 11.0 | nM | 7.96 |
| CHEMBL5981827 | — | IC50 | = | 17.0 | nM | 7.77 |
| CHEMBL5763295 | — | IC50 | = | 18.0 | nM | 7.75 |
| CHEMBL5850094 | — | IC50 | = | 18.0 | nM | 7.75 |
| CHEMBL6024336 | — | IC50 | = | 19.0 | nM | 7.72 |
| CHEMBL5838830 | — | IC50 | = | 19.0 | nM | 7.72 |
| CHEMBL5864393 | — | IC50 | = | 21.0 | nM | 7.68 |
| CHEMBL5746748 | — | IC50 | = | 21.0 | nM | 7.68 |
| CHEMBL5954175 | — | IC50 | = | 22.0 | nM | 7.66 |
| CHEMBL5747111 | — | IC50 | = | 24.0 | nM | 7.62 |
| CHEMBL5998021 | — | IC50 | = | 25.0 | nM | 7.60 |
| CHEMBL5840014 | — | IC50 | = | 26.0 | nM | 7.58 |
| CHEMBL5809280 | — | IC50 | = | 27.0 | nM | 7.57 |
| CHEMBL5948563 | — | IC50 | = | 28.0 | nM | 7.55 |
| CHEMBL6009283 | — | IC50 | = | 30.0 | nM | 7.52 |
| CHEMBL5892520 | — | IC50 | = | 31.0 | nM | 7.51 |
| CHEMBL5954639 | — | IC50 | = | 31.0 | nM | 7.51 |
| CHEMBL6038749 | — | IC50 | = | 31.0 | nM | 7.51 |
| CHEMBL5751647 | — | IC50 | = | 34.0 | nM | 7.47 |
| CHEMBL5913853 | — | IC50 | = | 36.0 | nM | 7.44 |
| CHEMBL5787808 | — | IC50 | = | 37.0 | nM | 7.43 |
| CHEMBL5940065 | — | IC50 | = | 42.0 | nM | 7.38 |
| CHEMBL5995237 | — | IC50 | = | 48.0 | nM | 7.32 |
| CHEMBL6053440 | — | IC50 | = | 49.0 | nM | 7.31 |
| CHEMBL5923936 | — | IC50 | = | 50.0 | nM | 7.30 |
| CHEMBL5986690 | — | IC50 | = | 50.0 | nM | 7.30 |
| CHEMBL5813018 | — | IC50 | = | 50.0 | nM | 7.30 |
| CHEMBL5931153 | — | IC50 | = | 51.0 | nM | 7.29 |
| CHEMBL6010352 | — | IC50 | = | 54.0 | nM | 7.27 |
| CHEMBL5946050 | — | IC50 | = | 59.0 | nM | 7.23 |
| CHEMBL5920371 | — | IC50 | = | 64.0 | nM | 7.19 |
| CHEMBL6000644 | — | IC50 | = | 65.0 | nM | 7.19 |
| CHEMBL5915403 | — | IC50 | = | 67.0 | nM | 7.17 |
| CHEMBL5956049 | — | IC50 | = | 69.0 | nM | 7.16 |
| CHEMBL5872061 | — | IC50 | = | 74.0 | nM | 7.13 |
| CHEMBL5915294 | — | IC50 | = | 74.0 | nM | 7.13 |
| CHEMBL5998589 | — | IC50 | = | 74.0 | nM | 7.13 |
| CHEMBL5901538 | — | IC50 | = | 75.0 | nM | 7.12 |
| CHEMBL5921155 | — | IC50 | = | 76.0 | nM | 7.12 |
| CHEMBL5839018 | — | IC50 | = | 75.0 | nM | 7.12 |
| CHEMBL5883482 | — | IC50 | = | 78.0 | nM | 7.11 |
| CHEMBL5771172 | — | IC50 | = | 79.0 | nM | 7.10 |
| CHEMBL5820000 | — | IC50 | = | 79.0 | nM | 7.10 |