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Assay Detail

CHEMBL5737065

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Automated Whole-Cell Patch Clamp Assay to Detect TMEM16A Activity in Recombinant Cells: Compound activity was quantified by measuring the increase in current upon compound addition and expressing this as a percentage increase of baseline TMEM16A current level. Percentage increases in current were determined for each concentration and the data plotted as a function of concentration using either the Qpatch software or Graphpad Prism v6.05 providing the concentration which gave 50% of its maximal effect (EC50) and maximum efficacy (percentage of baseline increase). The method of calculating the results is illustrated in FIG. 1, which shows an example trace from the Qpatch TMEM16A assay. In FIG. 1, IBL equals baseline current, I[#1] equals the peak current during test compound concentration 1 incubation period and so on.
699
Total Activities
228
Compounds Tested
3
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Anoctamin-1 (CHEMBL2046267)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

COMPOUNDS
(2020)

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
EC50 233 6.47 8.00
kon 233 - -
k_off 233 - -

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL5987751 3 8.00
CHEMBL5748528 3 8.00
CHEMBL5818335 3 8.00
CHEMBL5923346 3 7.70
CHEMBL5888912 3 7.70
CHEMBL6035973 3 7.70
CHEMBL6063625 3 7.70
CHEMBL6027216 3 7.70
CHEMBL5886634 3 7.40
CHEMBL5751293 3 7.40
CHEMBL5867107 3 7.40
CHEMBL5794087 3 7.40
CHEMBL5924873 3 7.30
CHEMBL5874757 3 7.30
CHEMBL5793913 3 7.30
CHEMBL5854301 3 7.30
CHEMBL5875064 3 7.30
CHEMBL5974117 3 7.22
CHEMBL5758425 3 7.22
CHEMBL5911037 3 7.22
CHEMBL6039401 3 7.22
CHEMBL5869137 3 7.16
CHEMBL6057591 3 7.16
CHEMBL5965278 3 7.10
CHEMBL5789360 3 7.05
CHEMBL5957346 3 7.05
CHEMBL6028707 3 7.05
CHEMBL6061522 3 7.00
CHEMBL5769067 6 7.00
CHEMBL5979945 3 7.00

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL5818335 EC50 = 10.0 nM 8.00
CHEMBL5987751 EC50 = 10.0 nM 8.00
CHEMBL5748528 EC50 = 10.0 nM 8.00
CHEMBL6035973 EC50 = 20.0 nM 7.70
CHEMBL6027216 EC50 = 20.0 nM 7.70
CHEMBL5888912 EC50 = 20.0 nM 7.70
CHEMBL5923346 EC50 = 20.0 nM 7.70
CHEMBL6063625 EC50 = 20.0 nM 7.70
CHEMBL5751293 EC50 = 40.0 nM 7.40
CHEMBL5867107 EC50 = 40.0 nM 7.40
CHEMBL5886634 EC50 = 40.0 nM 7.40
CHEMBL5794087 EC50 = 40.0 nM 7.40
CHEMBL5793913 EC50 = 50.0 nM 7.30
CHEMBL5874757 EC50 = 50.0 nM 7.30
CHEMBL5854301 EC50 = 50.0 nM 7.30
CHEMBL5875064 EC50 = 50.0 nM 7.30
CHEMBL5924873 EC50 = 50.0 nM 7.30
CHEMBL5911037 EC50 = 60.0 nM 7.22
CHEMBL5758425 EC50 = 60.0 nM 7.22
CHEMBL6039401 EC50 = 60.0 nM 7.22
CHEMBL5974117 EC50 = 60.0 nM 7.22
CHEMBL6057591 EC50 = 70.0 nM 7.16
CHEMBL5869137 EC50 = 70.0 nM 7.16
CHEMBL5965278 EC50 = 80.0 nM 7.10
CHEMBL6028707 EC50 = 90.0 nM 7.05
CHEMBL5957346 EC50 = 90.0 nM 7.05
CHEMBL5789360 EC50 = 90.0 nM 7.05
CHEMBL6043593 EC50 = 100.0 nM 7.00
CHEMBL5979945 EC50 = 100.0 nM 7.00
CHEMBL5769067 EC50 = 100.0 nM 7.00
CHEMBL6061522 EC50 = 100.0 nM 7.00
CHEMBL5912840 EC50 = 110.0 nM 6.96
CHEMBL5985276 EC50 = 110.0 nM 6.96
CHEMBL6055475 EC50 = 110.0 nM 6.96
CHEMBL6036293 EC50 = 120.0 nM 6.92
CHEMBL5872142 EC50 = 120.0 nM 6.92
CHEMBL5947400 EC50 = 120.0 nM 6.92
CHEMBL5822025 EC50 = 120.0 nM 6.92
CHEMBL5775948 EC50 = 120.0 nM 6.92
CHEMBL6061429 EC50 = 130.0 nM 6.89
CHEMBL5965194 EC50 = 130.0 nM 6.89
CHEMBL6061358 EC50 = 130.0 nM 6.89
CHEMBL6027996 EC50 = 140.0 nM 6.85
CHEMBL5751623 EC50 = 140.0 nM 6.85
CHEMBL5937186 EC50 = 140.0 nM 6.85
CHEMBL5944799 EC50 = 140.0 nM 6.85
CHEMBL5754879 EC50 = 140.0 nM 6.85
CHEMBL6033691 EC50 = 150.0 nM 6.82
CHEMBL5746392 EC50 = 150.0 nM 6.82
CHEMBL5928244 EC50 = 150.0 nM 6.82