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Assay Detail

CHEMBL5737147

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Binding
Electrophysiology: Electrophysiological experiments were performed on days 3 to 5 after the micro-injection of mRNA. During the experiment the oocytes were constantly superfused by a solution containing (in mM) NaCl 90, KCl 1, HEPES 5, MgCl2 1, CaCl2 1 (pH 7.4). Oocytes were impaled by two glass microelectrodes (resistance: 0.4 MΩ) which were filled with a solution containing KCl 1M+K-acetate 1.5 M and voltage-clamped to −80 mV. The recordings were performed at room temperature using the Roboocyte two-electrode voltage clamp system (Multichannelsystem). After an initial equilibration period of 1.5 min GABA was added for 1.5 min at a concentration evoking approximately 20% of a maximal current response (EC20). After another rest interval of 2.5 min GABA was again added evoking a response of similar amplitude and shape. 0.5 min after the onset of this second GABA application the test compound, at a concentration corresponding to approximatively 30 fold its Ki, was added while GABA was still present. Current traces were recorded at a digitization rate of 10 Hz during and shortly before and after the GABA application.
585
Total Activities
193
Compounds Tested
3
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Gamma-aminobutyric acid receptor subunit alpha-1 (CHEMBL1962)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Isoxazolyl ether derivatives as GABAA α5 PAM
(2021)

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
Ki 195 7.85 9.40
kon 195 - -
k_off 195 - -

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL5771253 3 9.40
CHEMBL6038200 3 9.10
CHEMBL6063116 3 9.00
CHEMBL5808514 3 8.92
CHEMBL6003049 3 8.85
CHEMBL6060527 3 8.62
CHEMBL5845026 3 8.59
CHEMBL6062914 3 8.52
CHEMBL5952606 3 8.46
CHEMBL5776143 3 8.36
CHEMBL6016406 3 8.34
CHEMBL5971033 3 8.34
CHEMBL5841019 3 8.33
CHEMBL5960872 3 8.32
CHEMBL5978694 3 8.31
CHEMBL5880547 3 8.30
CHEMBL5869614 3 8.30
CHEMBL5986198 3 8.28
CHEMBL6046793 3 8.28
CHEMBL5759279 3 8.28
CHEMBL5895042 3 8.28
CHEMBL6024468 3 8.27
CHEMBL5845333 3 8.26
CHEMBL5798454 3 8.26
CHEMBL6046677 3 8.24
CHEMBL5951376 3 8.24
CHEMBL6011117 3 8.22
CHEMBL5985273 3 8.21
CHEMBL5805665 3 8.21
CHEMBL5917239 3 8.20

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL5771253 Ki = 0.4 nM 9.40
CHEMBL6038200 Ki = 0.8 nM 9.10
CHEMBL6063116 Ki = 1.0 nM 9.00
CHEMBL5808514 Ki = 1.2 nM 8.92
CHEMBL6003049 Ki = 1.4 nM 8.85
CHEMBL6060527 Ki = 2.4 nM 8.62
CHEMBL5845026 Ki = 2.6 nM 8.59
CHEMBL6062914 Ki = 3.0 nM 8.52
CHEMBL5952606 Ki = 3.5 nM 8.46
CHEMBL5776143 Ki = 4.4 nM 8.36
CHEMBL5971033 Ki = 4.6 nM 8.34
CHEMBL6016406 Ki = 4.6 nM 8.34
CHEMBL5841019 Ki = 4.7 nM 8.33
CHEMBL5960872 Ki = 4.8 nM 8.32
CHEMBL5978694 Ki = 4.9 nM 8.31
CHEMBL5880547 Ki = 5.0 nM 8.30
CHEMBL5869614 Ki = 5.0 nM 8.30
CHEMBL5895042 Ki = 5.3 nM 8.28
CHEMBL5986198 Ki = 5.2 nM 8.28
CHEMBL6046793 Ki = 5.2 nM 8.28
CHEMBL5759279 Ki = 5.2 nM 8.28
CHEMBL6024468 Ki = 5.4 nM 8.27
CHEMBL5798454 Ki = 5.5 nM 8.26
CHEMBL5845333 Ki = 5.5 nM 8.26
CHEMBL5951376 Ki = 5.8 nM 8.24
CHEMBL6046677 Ki = 5.8 nM 8.24
CHEMBL6011117 Ki = 6.0 nM 8.22
CHEMBL5805665 Ki = 6.1 nM 8.21
CHEMBL5985273 Ki = 6.2 nM 8.21
CHEMBL5917239 Ki = 6.3 nM 8.20
CHEMBL5822054 Ki = 6.5 nM 8.19
CHEMBL5899241 Ki = 6.6 nM 8.18
CHEMBL6021494 Ki = 6.8 nM 8.17
CHEMBL5741267 Ki = 7.1 nM 8.15
CHEMBL5771391 Ki = 7.2 nM 8.14
CHEMBL5805945 Ki = 7.3 nM 8.14
CHEMBL6014829 Ki = 7.2 nM 8.14
CHEMBL5980072 Ki = 7.2 nM 8.14
CHEMBL6013523 Ki = 7.6 nM 8.12
CHEMBL5926555 Ki = 7.6 nM 8.12
CHEMBL5911837 Ki = 7.6 nM 8.12
CHEMBL5751783 Ki = 7.5 nM 8.12
CHEMBL5747907 Ki = 7.8 nM 8.11
CHEMBL6063739 Ki = 7.7 nM 8.11
CHEMBL5934990 Ki = 8.0 nM 8.10
CHEMBL5956891 Ki = 7.9 nM 8.10
CHEMBL5764050 Ki = 8.0 nM 8.10
CHEMBL5812257 Ki = 8.0 nM 8.10
CHEMBL5965541 Ki = 8.1 nM 8.09
CHEMBL5920931 Ki = 8.3 nM 8.08