Assay Detail
Binding
CHEMBL5737542
Review assay metadata, readout intent, target linkage, and publication context from the same page.
Biochemical EGFR Inhibition Assays (5 mM ATP): 5 μL of EGFR enzyme form in assay buffer (50 mM HEPES pH 7.3, 10 mM MgCl2, 1 mM EGTA, 0.01% Tween 20, 2 mM DTT) are dispensed into columns 1-23, than 5 μL of ATP and ULight-poly-GT substrate (PerkinElmer; TRF0100-M) mix in assay buffer is added to all wells (final assay concentration of the ULight-poly-GT substrate 200 nM). Each of the different EGFR enzyme form assays is available at low ATP (final assay concentration 5 μM). After 90 minutes incubation at room temperature 5 μL EDTA (final assay concentration 50 mM) and LANCE Eu-anti-P-Tyr (PT66) antibody (PerkinElmer, AD0069) (final assay concentration 6 nM) mix are added to stop the reaction and start the binding of the antibody. After additional 60 minutes incubation at room temperature the signal is measured in a PerkinElmer Envision HTS Multilabel Reader using the TR-FRET LANCE Ultra specs of PerkinElmer (used wavelengths: excitation 320 nm, emission 1 665 nm, emission 2 615 nm). Each plate contains 16 wells of a negative control (diluted DMSO instead of test compound; w EGFR enzyme form; column 23) and 16 wells of a positive control (diluted DMSO instead of test compound; w/o EGFR enzyme form; column 24). Negative and positive control values are used for normalization and IC50 values are calculated and analysed using a 4 parametric logistic model.
246
Total Activities
79
Compounds Tested
3
Activity Types
0
Assay Parameters
Assay Information
| Assay Type | Binding |
| Confidence | 8 — Homologous single protein target |
| Curated By | Autocuration |
Publication
Benzimidazole compounds and derivatives as EGFR inhibitors
Activity Statistics
| Type | Count | Avg pChEMBL | Best pChEMBL |
|---|---|---|---|
| IC50 | 82 | 6.32 | 7.90 |
| kon | 82 | - | - |
| k_off | 82 | - | - |
Compounds Tested
| Compound | Name | Phase | Activities | Best pChEMBL |
|---|---|---|---|---|
| CHEMBL5932830 | — | — | 3 | 7.90 |
| CHEMBL5790363 | — | — | 3 | 7.71 |
| CHEMBL5755058 | — | — | 3 | 7.59 |
| CHEMBL6057006 | — | — | 3 | 7.51 |
| CHEMBL6020978 | — | — | 3 | 7.50 |
| CHEMBL6029813 | — | — | 3 | 7.49 |
| CHEMBL5756022 | — | — | 3 | 7.46 |
| CHEMBL5887634 | — | — | 3 | 7.43 |
| CHEMBL5969655 | — | — | 3 | 7.25 |
| CHEMBL5788210 | — | — | 3 | 7.18 |
| CHEMBL5893107 | — | — | 3 | 7.15 |
| CHEMBL5965573 | — | — | 3 | 7.11 |
| CHEMBL5998521 | — | — | 3 | 7.11 |
| CHEMBL5880562 | — | — | 3 | 7.09 |
| CHEMBL5843126 | — | — | 6 | 6.98 |
| CHEMBL5758176 | — | — | 3 | 6.96 |
| CHEMBL5772876 | — | — | 3 | 6.96 |
| CHEMBL5825830 | — | — | 3 | 6.90 |
| CHEMBL6050361 | — | — | 3 | 6.83 |
| CHEMBL5969707 | — | — | 3 | 6.79 |
| CHEMBL6043073 | — | — | 6 | 6.79 |
| CHEMBL5920247 | — | — | 3 | 6.76 |
| CHEMBL6057405 | — | — | 3 | 6.75 |
| CHEMBL5959712 | — | — | 3 | 6.73 |
| CHEMBL5996878 | — | — | 3 | 6.69 |
| CHEMBL5786240 | — | — | 3 | 6.67 |
| CHEMBL6027769 | — | — | 3 | 6.65 |
| CHEMBL5800758 | — | — | 3 | 6.64 |
| CHEMBL5832595 | — | — | 3 | 6.60 |
| CHEMBL5993712 | — | — | 3 | 6.57 |
Activity Data
| Compound | Name | Type | Rel. | Value | Units | pChEMBL |
|---|---|---|---|---|---|---|
| CHEMBL5932830 | — | IC50 | = | 12.5 | nM | 7.90 |
| CHEMBL5790363 | — | IC50 | = | 19.3 | nM | 7.71 |
| CHEMBL5755058 | — | IC50 | = | 25.8 | nM | 7.59 |
| CHEMBL6057006 | — | IC50 | = | 31.0 | nM | 7.51 |
| CHEMBL6020978 | — | IC50 | = | 31.5 | nM | 7.50 |
| CHEMBL6029813 | — | IC50 | = | 32.3 | nM | 7.49 |
| CHEMBL5756022 | — | IC50 | = | 34.5 | nM | 7.46 |
| CHEMBL5887634 | — | IC50 | = | 37.4 | nM | 7.43 |
| CHEMBL5969655 | — | IC50 | = | 56.3 | nM | 7.25 |
| CHEMBL5788210 | — | IC50 | = | 65.6 | nM | 7.18 |
| CHEMBL5893107 | — | IC50 | = | 70.4 | nM | 7.15 |
| CHEMBL5965573 | — | IC50 | = | 77.1 | nM | 7.11 |
| CHEMBL5998521 | — | IC50 | = | 77.8 | nM | 7.11 |
| CHEMBL5880562 | — | IC50 | = | 81.6 | nM | 7.09 |
| CHEMBL5843126 | — | IC50 | = | 104.0 | nM | 6.98 |
| CHEMBL5843126 | — | IC50 | = | 108.0 | nM | 6.97 |
| CHEMBL5772876 | — | IC50 | = | 110.0 | nM | 6.96 |
| CHEMBL5758176 | — | IC50 | = | 110.0 | nM | 6.96 |
| CHEMBL5825830 | — | IC50 | = | 125.0 | nM | 6.90 |
| CHEMBL6050361 | — | IC50 | = | 148.0 | nM | 6.83 |
| CHEMBL6043073 | — | IC50 | = | 164.0 | nM | 6.79 |
| CHEMBL5969707 | — | IC50 | = | 162.0 | nM | 6.79 |
| CHEMBL5920247 | — | IC50 | = | 173.0 | nM | 6.76 |
| CHEMBL6057405 | — | IC50 | = | 179.0 | nM | 6.75 |
| CHEMBL5959712 | — | IC50 | = | 185.0 | nM | 6.73 |
| CHEMBL5996878 | — | IC50 | = | 205.0 | nM | 6.69 |
| CHEMBL5786240 | — | IC50 | = | 213.0 | nM | 6.67 |
| CHEMBL6027769 | — | IC50 | = | 225.0 | nM | 6.65 |
| CHEMBL5800758 | — | IC50 | = | 231.0 | nM | 6.64 |
| CHEMBL5832595 | — | IC50 | = | 249.0 | nM | 6.60 |
| CHEMBL6043073 | — | IC50 | = | 256.0 | nM | 6.59 |
| CHEMBL5993712 | — | IC50 | = | 267.0 | nM | 6.57 |
| CHEMBL5785378 | — | IC50 | = | 293.0 | nM | 6.53 |
| CHEMBL5812562 | — | IC50 | = | 302.0 | nM | 6.52 |
| CHEMBL5861063 | — | IC50 | = | 305.0 | nM | 6.52 |
| CHEMBL5838821 | — | IC50 | = | 312.0 | nM | 6.51 |
| CHEMBL6060232 | — | IC50 | = | 318.0 | nM | 6.50 |
| CHEMBL6023158 | — | IC50 | = | 318.0 | nM | 6.50 |
| CHEMBL6007714 | — | IC50 | = | 336.0 | nM | 6.47 |
| CHEMBL5749996 | — | IC50 | = | 397.0 | nM | 6.40 |
| CHEMBL5893361 | — | IC50 | = | 403.0 | nM | 6.39 |
| CHEMBL5195167 | — | IC50 | = | 407.0 | nM | 6.39 |
| CHEMBL6025269 | — | IC50 | = | 439.0 | nM | 6.36 |
| CHEMBL5770970 | — | IC50 | = | 539.0 | nM | 6.27 |
| CHEMBL5759154 | — | IC50 | = | 557.0 | nM | 6.25 |
| CHEMBL5905751 | — | IC50 | = | 664.0 | nM | 6.18 |
| CHEMBL5861063 | — | IC50 | = | 777.0 | nM | 6.11 |
| CHEMBL5870210 | — | IC50 | = | 958.0 | nM | 6.02 |
| CHEMBL5956362 | — | IC50 | = | 995.0 | nM | 6.00 |
| CHEMBL5894059 | — | IC50 | = | 1128.0 | nM | 5.95 |