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Assay Detail

CHEMBL5738104

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Binding
Inhibitory Activity for hERG Channel: Extracellular and intracellular fluids used in the measurement were prepared as follows.Extracellular fluid: 2 mmol/L CaCl2, 1 mmol/L MgCl2, 10 mmol/L HEPES, 4 mmol/L KCl, 145 mmol/L NaCl, 10 mmol/L GlucoseIntracellular fluid: 10 mmol/L HEPES, 10 mmol/L EGTA, 20 mmol/L KCl, 130 mmol/L KFTest compound solution: A test compound was dissolved in DMSO to reach the concentration of 2 mmol/L or 20 mmol/L in order to prepare a test compound solution. The test compound solution was further diluted 200 times with the extracellular fluid, and serial-diluted with the extracellular fluid to prepare a test compound solution in each concentration which is necessary to calculate IC50 value of hERG inhibition.
207
Total Activities
68
Compounds Tested
3
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Publication

Aliphatic acid amide derivative
(2022)

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 69 5.34 6.05
kon 69 - -
k_off 69 - -

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL5958853 3 6.05
CHEMBL6059801 3 5.96
CHEMBL5965478 3 5.92
CHEMBL5975258 3 5.89
CHEMBL5804136 3 5.82
CHEMBL6011569 3 5.68
CHEMBL5798443 3 5.66
CHEMBL5905387 3 5.66
CHEMBL5924032 3 5.55
CHEMBL5795064 3 5.54
CHEMBL5871355 3 5.54
CHEMBL6065133 3 5.51
CHEMBL5974043 3 5.46
CHEMBL5890912 3 5.44
CHEMBL5959979 3 5.42
CHEMBL5844582 3 5.39
CHEMBL6014765 3 5.38
CHEMBL6062980 3 5.38
CHEMBL5800878 3 5.37
CHEMBL5892765 3 5.33
CHEMBL5844083 3 5.32
CHEMBL5753449 3 5.32
CHEMBL5908106 3 5.27
CHEMBL5991784 3 5.27
CHEMBL5836417 3 5.27
CHEMBL5827826 3 5.26
CHEMBL5768736 3 5.24
CHEMBL6001447 3 5.21
CHEMBL5862224 3 5.21
CHEMBL6062801 3 5.19

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL5958853 IC50 = 900.0 nM 6.05
CHEMBL6059801 IC50 = 1100.0 nM 5.96
CHEMBL5965478 IC50 = 1200.0 nM 5.92
CHEMBL5975258 IC50 = 1300.0 nM 5.89
CHEMBL5804136 IC50 = 1500.0 nM 5.82
CHEMBL6011569 IC50 = 2100.0 nM 5.68
CHEMBL5798443 IC50 = 2200.0 nM 5.66
CHEMBL5905387 IC50 = 2200.0 nM 5.66
CHEMBL5924032 IC50 = 2800.0 nM 5.55
CHEMBL5795064 IC50 = 2900.0 nM 5.54
CHEMBL5871355 IC50 = 2900.0 nM 5.54
CHEMBL6065133 IC50 = 3100.0 nM 5.51
CHEMBL5974043 IC50 = 3500.0 nM 5.46
CHEMBL5890912 IC50 = 3600.0 nM 5.44
CHEMBL5959979 IC50 = 3800.0 nM 5.42
CHEMBL5844582 IC50 = 4100.0 nM 5.39
CHEMBL6062980 IC50 = 4200.0 nM 5.38
CHEMBL6014765 IC50 = 4200.0 nM 5.38
CHEMBL5800878 IC50 = 4300.0 nM 5.37
CHEMBL5892765 IC50 = 4700.0 nM 5.33
CHEMBL5844083 IC50 = 4800.0 nM 5.32
CHEMBL5753449 IC50 = 4800.0 nM 5.32
CHEMBL5991784 IC50 = 5400.0 nM 5.27
CHEMBL5836417 IC50 = 5400.0 nM 5.27
CHEMBL5908106 IC50 = 5400.0 nM 5.27
CHEMBL5827826 IC50 = 5500.0 nM 5.26
CHEMBL5768736 IC50 = 5700.0 nM 5.24
CHEMBL5862224 IC50 = 6200.0 nM 5.21
CHEMBL6001447 IC50 = 6200.0 nM 5.21
CHEMBL6062801 IC50 = 6400.0 nM 5.19
CHEMBL6026858 IC50 = 6400.0 nM 5.19
CHEMBL5827131 IC50 = 6700.0 nM 5.17
CHEMBL5982627 IC50 = 6700.0 nM 5.17
CHEMBL5978090 IC50 = 7000.0 nM 5.16
CHEMBL5787400 IC50 = 6900.0 nM 5.16
CHEMBL5928632 IC50 = 7100.0 nM 5.15
CHEMBL5882802 IC50 = 7200.0 nM 5.14
CHEMBL6033170 IC50 = 7300.0 nM 5.14
CHEMBL5789483 IC50 = 7400.0 nM 5.13
CHEMBL5889007 IC50 = 7400.0 nM 5.13
CHEMBL5889491 IC50 = 7400.0 nM 5.13
CHEMBL5940672 IC50 = 9600.0 nM 5.02
CHEMBL5774136 IC50 = 9900.0 nM 5.00
CHEMBL5928632 IC50 = 9900.0 nM 5.00
CHEMBL5758329 IC50 = 10500.0 nM 4.98
CHEMBL5888503 IC50 = 14500.0 nM 4.84
CHEMBL5953904 IC50 = 15300.0 nM 4.82
CHEMBL6021264 IC50 > 10000.0 nM -
CHEMBL5889491 kon = - -
CHEMBL5889491 k_off = - s-1 -