Assay Detail
Binding
CHEMBL5738752
Review assay metadata, readout intent, target linkage, and publication context from the same page.
DNMT1 Inhibition Assay: The DNMT1 assays were carried to determine the IC50 with transition state analogs. The reaction (100 μL) containing 50 mM Tris-HCl pH 8.0, 100 mM KCl, 50 ug/mL BSA, 2 mM DTT, 1 μM freshly prepared [Me-3H]-SAM, 1 μM 26 bp hemimethylated DNA substrate and varying concentrations of each inhibitor (0-100 1 μM), incubated at 37° C. with 100 nM DNMT1. The reaction fractions (50 μL) were taken at 2 hours. The methylated DNA was purified by using Micro Bio-spin P-30 columns (Bio-Rad). Purified samples were mixed with 10 mL of scintillation liquid (PerkinElmer), and counted for radioactivity in a Tricarb 2910 TR scintillation counter (PerkinElmer). The values obtained in the absence of DNMT1 in the reaction were used as controls. The assay data were analyzed using GraphPad Prism for IC50 calculations.
24
Total Activities
8
Compounds Tested
3
Activity Types
0
Assay Parameters
Assay Information
| Assay Type | Binding |
| Confidence | 8 — Homologous single protein target |
| Curated By | Autocuration |
Publication
Inhibitors of DNMT1 as anticancer therapeutic agents
Activity Statistics
| Type | Count | Avg pChEMBL | Best pChEMBL |
|---|---|---|---|
| IC50 | 8 | 5.63 | 6.16 |
| kon | 8 | - | - |
| k_off | 8 | - | - |
Compounds Tested
| Compound | Name | Phase | Activities | Best pChEMBL |
|---|---|---|---|---|
| CHEMBL5963195 | — | — | 3 | 6.16 |
| CHEMBL5868689 | — | — | 3 | 6.10 |
| CHEMBL5187655 | — | — | 3 | 5.96 |
| CHEMBL5746485 | — | — | 3 | 5.72 |
| CHEMBL5849152 | — | — | 3 | 5.66 |
| CHEMBL5189142 | — | — | 3 | 5.44 |
| CHEMBL5843176 | — | — | 3 | 5.22 |
| CHEMBL6058668 | — | — | 3 | 4.81 |
Activity Data
| Compound | Name | Type | Rel. | Value | Units | pChEMBL |
|---|---|---|---|---|---|---|
| CHEMBL5963195 | — | IC50 | = | 700.0 | nM | 6.16 |
| CHEMBL5868689 | — | IC50 | = | 800.0 | nM | 6.10 |
| CHEMBL5187655 | — | IC50 | = | 1100.0 | nM | 5.96 |
| CHEMBL5746485 | — | IC50 | = | 1900.0 | nM | 5.72 |
| CHEMBL5849152 | — | IC50 | = | 2200.0 | nM | 5.66 |
| CHEMBL5189142 | — | IC50 | = | 3600.0 | nM | 5.44 |
| CHEMBL5843176 | — | IC50 | = | 6000.0 | nM | 5.22 |
| CHEMBL6058668 | — | IC50 | = | 15400.0 | nM | 4.81 |
| CHEMBL5963195 | — | kon | = | - | — | - |
| CHEMBL5963195 | — | k_off | = | - | s-1 | - |
| CHEMBL5187655 | — | kon | = | - | — | - |
| CHEMBL5187655 | — | k_off | = | - | s-1 | - |
| CHEMBL5189142 | — | kon | = | - | — | - |
| CHEMBL5189142 | — | k_off | = | - | s-1 | - |
| CHEMBL5868689 | — | kon | = | - | — | - |
| CHEMBL5868689 | — | k_off | = | - | s-1 | - |
| CHEMBL6058668 | — | kon | = | - | — | - |
| CHEMBL6058668 | — | k_off | = | - | s-1 | - |
| CHEMBL5746485 | — | kon | = | - | — | - |
| CHEMBL5746485 | — | k_off | = | - | s-1 | - |
| CHEMBL5849152 | — | kon | = | - | — | - |
| CHEMBL5849152 | — | k_off | = | - | s-1 | - |
| CHEMBL5843176 | — | kon | = | - | — | - |
| CHEMBL5843176 | — | k_off | = | - | s-1 | - |