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Assay Detail

CHEMBL617043

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Functional
Agonist-induced [35S]GTP-gamma-S, binding in CHO cells stably transfected with human 5-hydroxytryptamine 1D receptor
15
Total Activities
15
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Functional
Cell Type CHO
Confidence 8 — Homologous single protein target
Curated By Expert

Target

5-hydroxytryptamine receptor 1D (CHEMBL1983)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Fluorination of 3-(3-(piperidin-1-yl)propyl)indoles and 3-(3-(piperazin-1-yl)propyl)indoles gives selective human 5-HT1D receptor ligands with improved pharmacokinetic profiles.
van Niel MB, Collins I, Beer MS, Broughton HB, Cheng SK, Goodacre SC, Heald A, Locker KL, MacLeod AM, Morrison D, Moyes CR, O'Connor D, Pike A, Rowley M, Russell MG, Sohal B, Stanton JA, Thomas S, Verrier H, Watt AP, Castro JL.
J Med Chem (1999) 42 : 2087 -2104
PubMed 10377215 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
EC50 15 8.57 9.70

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL63167 1 9.70
CHEMBL294732 1 9.40
CHEMBL294547 1 9.22
CHEMBL432332 1 9.05
CHEMBL292779 1 9.00
CHEMBL304857 1 8.82
CHEMBL64004 1 8.80
CHEMBL65321 1 8.72
CHEMBL67684 1 8.59
CHEMBL63432 1 8.42
CHEMBL63799 1 8.30
CHEMBL433108 1 7.80
CHEMBL416688 1 7.75
CHEMBL63258 1 7.47
CHEMBL305301 1 7.46

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL63167 EC50 = 0.2 nM 9.70
CHEMBL294732 EC50 = 0.4 nM 9.40
CHEMBL294547 EC50 = 0.6 nM 9.22
CHEMBL432332 EC50 = 0.9 nM 9.05
CHEMBL292779 EC50 = 1.0 nM 9.00
CHEMBL304857 EC50 = 1.5 nM 8.82
CHEMBL64004 EC50 = 1.6 nM 8.80
CHEMBL65321 EC50 = 1.9 nM 8.72
CHEMBL67684 EC50 = 2.6 nM 8.59
CHEMBL63432 EC50 = 3.8 nM 8.42
CHEMBL63799 EC50 = 5.0 nM 8.30
CHEMBL433108 EC50 = 16.0 nM 7.80
CHEMBL416688 EC50 = 18.0 nM 7.75
CHEMBL63258 EC50 = 34.0 nM 7.47
CHEMBL305301 EC50 = 35.0 nM 7.46