Assay Detail
Binding
CHEMBL618052
Review assay metadata, readout intent, target linkage, and publication context from the same page.
Inhibition of 5-lipoxygenase from rat basophilic leukemia(RBL-1) cells
12
Total Activities
12
Compounds Tested
1
Activity Types
0
Assay Parameters
Assay Information
| Assay Type | Binding |
| Organism | Rattus norvegicus |
| Cell Type | RBL-1 |
| Confidence | 9 — Direct single protein target |
| Curated By | Expert |
Target
Polyunsaturated fatty acid 5-lipoxygenase (CHEMBL312) ↗| Type | SINGLE PROTEIN |
| Organism | Rattus norvegicus |
Publication
4-[5-(2,3-dihydroxyphenyl)pentyloxy]-2-hydroxy-3-propylbenzoic acid (Ro 24-0553): an orally active 5-lipoxygenase inhibitor with antiinflammatory activity
Activity Statistics
| Type | Count | Avg pChEMBL | Best pChEMBL |
|---|---|---|---|
| IC50 | 12 | 7.36 | 8.30 |
Compounds Tested
| Compound | Name | Phase | Activities | Best pChEMBL |
|---|---|---|---|---|
| CHEMBL114516 | — | — | 1 | 8.30 |
| CHEMBL116078 | — | — | 1 | 8.22 |
| CHEMBL52 | NORDIHYDROGUAIARETIC ACID | — | 1 | 8.00 |
| CHEMBL115813 | — | — | 1 | 7.70 |
| CHEMBL114013 | — | — | 1 | 7.70 |
| CHEMBL116755 | — | — | 1 | 7.70 |
| CHEMBL116201 | — | — | 1 | 7.05 |
| CHEMBL113700 | — | — | 1 | 7.05 |
| CHEMBL113768 | — | — | 1 | 6.77 |
| CHEMBL117203 | — | — | 1 | 6.70 |
| CHEMBL116496 | — | — | 1 | 6.60 |
| CHEMBL116612 | — | — | 1 | 6.52 |
Activity Data
| Compound | Name | Type | Rel. | Value | Units | pChEMBL |
|---|---|---|---|---|---|---|
| CHEMBL114516 | — | IC50 | = | 5.0 | nM | 8.30 |
| CHEMBL116078 | — | IC50 | = | 6.0 | nM | 8.22 |
| CHEMBL52 | NORDIHYDROGUAIARETIC ACID | IC50 | = | 10.0 | nM | 8.00 |
| CHEMBL115813 | — | IC50 | = | 20.0 | nM | 7.70 |
| CHEMBL114013 | — | IC50 | = | 20.0 | nM | 7.70 |
| CHEMBL116755 | — | IC50 | = | 20.0 | nM | 7.70 |
| CHEMBL116201 | — | IC50 | = | 90.0 | nM | 7.05 |
| CHEMBL113700 | — | IC50 | = | 90.0 | nM | 7.05 |
| CHEMBL113768 | — | IC50 | = | 170.0 | nM | 6.77 |
| CHEMBL117203 | — | IC50 | = | 200.0 | nM | 6.70 |
| CHEMBL116496 | — | IC50 | = | 250.0 | nM | 6.60 |
| CHEMBL116612 | — | IC50 | = | 300.0 | nM | 6.52 |