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Assay Detail

CHEMBL618927

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Functional
5-hydroxytryptamine 4 receptor agonist activity as increased response to electrical stimulation in guinea pig ileum
10
Total Activities
10
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Functional
Organism Cavia porcellus
Tissue Ileum
Confidence 9 — Direct single protein target
Curated By Intermediate

Target

5-hydroxytryptamine receptor 4 (CHEMBL5017)
Type SINGLE PROTEIN
Organism Cavia porcellus

Publication

Design of a potent 5-HT4 receptor agonist with nanomolar affinity
Langlois M, Zhang L, Bre mont B, Shen S, Manara L, Croci T
Bioorg Med Chem Lett (1994) 4 : 1433 -1436
· DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
EC50 10 7.40 8.40

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL286136 1 8.40
CHEMBL39 SEROTONIN 3.0 1 8.30
CHEMBL33732 1 7.66
CHEMBL33676 1 7.57
CHEMBL32986 1 7.36
CHEMBL34224 1 6.96
CHEMBL41769 1 6.96
CHEMBL288199 1 6.82
CHEMBL18041 ZACOPRIDE 2.0 1 6.58
CHEMBL287045 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL286136 EC50 = 4.0 nM 8.40
CHEMBL39 SEROTONIN EC50 = 5.0 nM 8.30
CHEMBL33732 EC50 = 22.0 nM 7.66
CHEMBL33676 EC50 = 27.0 nM 7.57
CHEMBL32986 EC50 = 44.0 nM 7.36
CHEMBL34224 EC50 = 110.0 nM 6.96
CHEMBL41769 EC50 = 110.0 nM 6.96
CHEMBL288199 EC50 = 153.0 nM 6.82
CHEMBL18041 ZACOPRIDE EC50 = 260.0 nM 6.58
CHEMBL287045 EC50 > 1000.0 nM -