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Assay Detail

CHEMBL619789

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibitory activity against 5-hydroxytryptamine 3 receptor in rat cortical membranes using [3H]- 1-Methyl-1H-indazole-3-carboxylic acid (8-methyl-8-aza-bicyclo[3.2.1]oct-3-yl)-amide as a radioligand
8
Total Activities
8
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Confidence 6 — Multiple protein complex / RNA
Curated By Autocuration

Target

Serotonin 3 (5-HT3) receptor (CHEMBL2094116)
Type PROTEIN COMPLEX
Organism Rattus norvegicus

Publication

Synthesis and biochemical evaluation of tritium-labeled 1-methyl-N-(8-methyl-8-azabicyclo[3.2.1]oct-3-yl)-1H-indazole-3-carboxa mide, a useful radioligand for 5HT3 receptors.
Robertson DW, Bloomquist W, Cohen ML, Reid LR, Schenck K, Wong DT.
J Med Chem (1990) 33 : 3176 -3181
PubMed 2258903 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
Ki 8 7.98 9.38

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL18041 ZACOPRIDE 2.0 1 9.38
CHEMBL56564 TROPISETRON 3.0 1 8.89
CHEMBL289469 GRANISETRON 4.0 1 8.79
CHEMBL18772 QUIPAZINE 2.0 1 8.52
CHEMBL38582 1 8.37
CHEMBL2107804 BEMESETRON 2.0 1 7.58
CHEMBL86 METOCLOPRAMIDE 4.0 1 6.30
CHEMBL39109 1 5.98

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL18041 ZACOPRIDE Ki = 0.42 nM 9.38
CHEMBL56564 TROPISETRON Ki = 1.28 nM 8.89
CHEMBL289469 GRANISETRON Ki = 1.62 nM 8.79
CHEMBL18772 QUIPAZINE Ki = 3.04 nM 8.52
CHEMBL38582 Ki = 4.26 nM 8.37
CHEMBL2107804 BEMESETRON Ki = 26.05 nM 7.58
CHEMBL86 METOCLOPRAMIDE Ki = 502.0 nM 6.30
CHEMBL39109 Ki = 1048.0 nM 5.98