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Assay Detail

CHEMBL636731

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Evaluated for the binding affinity towards the Adenosine A1 receptor in cerebral cortices of Sprague-Dawley male rats using [3H]CHA as radioligand.
5
Total Activities
5
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Confidence 8 — Homologous single protein target
Curated By Expert

Target

Adenosine receptor A1 (CHEMBL318)
Type SINGLE PROTEIN
Organism Rattus norvegicus

Publication

4-Amino[1,2,4]triazolo[4,3-a]quinoxalines. A novel class of potent adenosine receptor antagonists and potential rapid-onset antidepressants.
Sarges R, Howard HR, Browne RG, Lebel LA, Seymour PA, Koe BK.
J Med Chem (1990) 33 : 2240 -2254
PubMed 2374150 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 5 7.08 8.62

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL139000 (R)-PIA 1 8.62
CHEMBL262083 N6-PHENYLADENOSINE 1 8.19
CHEMBL420705 (S)-PIA 1 6.98
CHEMBL68738 N6-CYCLOPENTYLADENOSINE 1 6.19
CHEMBL260167 1 5.43

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL139000 (R)-PIA IC50 = 2.4 nM 8.62
CHEMBL262083 N6-PHENYLADENOSINE IC50 = 6.5 nM 8.19
CHEMBL420705 (S)-PIA IC50 = 105.0 nM 6.98
CHEMBL68738 N6-CYCLOPENTYLADENOSINE IC50 = 640.0 nM 6.19
CHEMBL260167 IC50 = 3700.0 nM 5.43