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Assay Detail

CHEMBL642021

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Tested for the binding affinity of A3 receptor by displacing N6-[[125I]-4-amino-3-iodobenzyl]-adenosine-5''-N-methyluronamide from membranes of CHO cells transfected with rat A3-cDNA
15
Total Activities
15
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Cell Type CHO
Confidence 8 — Homologous single protein target
Curated By Expert

Target

Adenosine receptor A3 (CHEMBL3360)
Type SINGLE PROTEIN
Organism Rattus norvegicus

Publication

2-Substitution of N6-benzyladenosine-5'-uronamides enhances selectivity for A3 adenosine receptors.
Kim HO, Ji XD, Siddiqi SM, Olah ME, Stiles GL, Jacobson KA.
J Med Chem (1994) 37 : 3614 -3621
PubMed 7932588 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
Ki 15 7.69 9.48

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL431733 NAMODENOSON 3.0 1 9.48
CHEMBL119709 IB-MECA 1 8.96
CHEMBL133566 1 8.89
CHEMBL307199 1 8.85
CHEMBL2113557 1 8.64
CHEMBL2113562 1 8.51
CHEMBL66393 1 8.02
CHEMBL609534 1 7.81
CHEMBL1950647 1 7.30
CHEMBL1256714 1 6.94
CHEMBL2113561 1 6.74
CHEMBL284969 1 6.62
CHEMBL68738 N6-CYCLOPENTYLADENOSINE 1 6.62
CHEMBL331372 CGS-21680 1 6.23
CHEMBL285819 1 5.72

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL431733 NAMODENOSON Ki = 0.33 nM 9.48
CHEMBL119709 IB-MECA Ki = 1.1 nM 8.96
CHEMBL133566 Ki = 1.3 nM 8.89
CHEMBL307199 Ki = 1.41 nM 8.85
CHEMBL2113557 Ki = 2.3 nM 8.64
CHEMBL2113562 Ki = 3.12 nM 8.51
CHEMBL66393 Ki = 9.5 nM 8.02
CHEMBL609534 Ki = 15.5 nM 7.81
CHEMBL1950647 Ki = 50.0 nM 7.30
CHEMBL1256714 Ki = 116.0 nM 6.94
CHEMBL2113561 Ki = 181.0 nM 6.74
CHEMBL284969 Ki = 237.0 nM 6.62
CHEMBL68738 N6-CYCLOPENTYLADENOSINE Ki = 240.0 nM 6.62
CHEMBL331372 CGS-21680 Ki = 584.0 nM 6.23
CHEMBL285819 Ki = 1890.0 nM 5.72