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Assay Detail

CHEMBL642986

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Binding affinity towards adenosine A1 receptor in rat forebrain membranes using N6-[3H]cyclohexyladenosine
4
Total Activities
4
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Rattus norvegicus
Tissue Forebrain
Subcellular Membrane
Confidence 9 — Direct single protein target
Curated By Expert

Target

Adenosine receptor A1 (CHEMBL318)
Type SINGLE PROTEIN
Organism Rattus norvegicus

Publication

Adenosine A1 antagonists. 2. Structure-activity relationships on diuretic activities and protective effects against acute renal failure.
Suzuki F, Shimada J, Mizumoto H, Karasawa A, Kubo K, Nonaka H, Ishii A, Kawakita T.
J Med Chem (1992) 35 : 3066 -3075
PubMed 1501234 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
Ki 4 8.26 9.72

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL52333 ROLOFYLLINE 3.0 1 9.72
CHEMBL183 1,3-DIPROPYL-8-CYCLOPENTYLXANTHINE [DPCPX] 1 9.31
CHEMBL84363 1 9.00
CHEMBL1355736 THEOPHYLLINE 4.0 1 5.01

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL52333 ROLOFYLLINE Ki = 0.19 nM 9.72
CHEMBL183 1,3-DIPROPYL-8-CYCLOPENTYLXANTHINE [DPCPX] Ki = 0.49 nM 9.31
CHEMBL84363 Ki = 0.99 nM 9.00
CHEMBL1355736 THEOPHYLLINE Ki = 9700.0 nM 5.01