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Assay Detail

CHEMBL642988

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Binding affinity towards adenosine A1 receptor on rat whole brain membrane using [3H]N6-cyclohexyladenosine
16
Total Activities
16
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Tissue Brain
Subcellular Membrane
Confidence 8 — Homologous single protein target
Curated By Expert

Target

Adenosine receptor A1 (CHEMBL318)
Type SINGLE PROTEIN
Organism Rattus norvegicus

Publication

N6-(arylalkyl)adenosines. Identification of N6-(9-fluorenylmethyl)adenosine as a highly potent agonist for the adenosine A2 receptor.
Trivedi BK, Bristol JA, Bruns RF, Haleen SJ, Steffen RP.
J Med Chem (1988) 31 : 271 -273
PubMed 3336027 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
Ki 16 7.39 9.23

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL68738 N6-CYCLOPENTYLADENOSINE 1 9.23
CHEMBL139000 (R)-PIA 1 8.92
CHEMBL2181971 1 8.29
CHEMBL464859 NECA 1 8.20
CHEMBL193692 1 8.17
CHEMBL285819 1 8.03
CHEMBL604617 1 7.91
CHEMBL1257013 1 7.89
CHEMBL132960 1 7.62
CHEMBL604967 1 7.21
CHEMBL388757 N6-BENZYLADENOSINE 1 6.92
CHEMBL609501 1 6.80
CHEMBL609790 1 6.50
CHEMBL1256672 1 6.23
CHEMBL604961 1 5.21
CHEMBL606227 1 5.05

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL68738 N6-CYCLOPENTYLADENOSINE Ki = 0.59 nM 9.23
CHEMBL139000 (R)-PIA Ki = 1.2 nM 8.92
CHEMBL2181971 Ki = 5.1 nM 8.29
CHEMBL464859 NECA Ki = 6.3 nM 8.20
CHEMBL193692 Ki = 6.8 nM 8.17
CHEMBL285819 Ki = 9.3 nM 8.03
CHEMBL604617 Ki = 12.3 nM 7.91
CHEMBL1257013 Ki = 13.0 nM 7.89
CHEMBL132960 Ki = 24.0 nM 7.62
CHEMBL604967 Ki = 62.0 nM 7.21
CHEMBL388757 N6-BENZYLADENOSINE Ki = 120.0 nM 6.92
CHEMBL609501 Ki = 160.0 nM 6.80
CHEMBL609790 Ki = 320.0 nM 6.50
CHEMBL1256672 Ki = 590.0 nM 6.23
CHEMBL604961 Ki = 6200.0 nM 5.21
CHEMBL606227 Ki = 9000.0 nM 5.05