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Assay Detail

CHEMBL643127

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Binding affinity determined by displacement of specific binding of [125I]N-(4-amino-3-iodophenethyl)-adenosine in membranes of CHO cells stably transfected with the rat adenosine A3 receptor
29
Total Activities
29
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Cell Type CHO
Confidence 8 — Homologous single protein target
Curated By Expert

Target

Adenosine receptor A3 (CHEMBL3360)
Type SINGLE PROTEIN
Organism Rattus norvegicus

Publication

Search for new purine- and ribose-modified adenosine analogues as selective agonists and antagonists at adenosine receptors.
Siddiqi SM, Jacobson KA, Esker JL, Olah ME, Ji XD, Melman N, Tiwari KN, Secrist JA, Schneller SW, Cristalli G.
J Med Chem (1995) 38 : 1174 -1188
PubMed 7707320 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
Ki 29 5.77 8.96

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL119709 IB-MECA 1 8.96
CHEMBL49843 1 7.80
CHEMBL410873 1 7.59
CHEMBL464859 NECA 1 6.95
CHEMBL388757 N6-BENZYLADENOSINE 1 6.92
CHEMBL331382 METRIFUDIL 2.0 1 6.44
CHEMBL2113395 1 6.22
CHEMBL2113396 1 6.15
CHEMBL2113475 1 6.14
CHEMBL411066 KINETIN RIBOSIDE 1 6.13
CHEMBL2079643 1 5.96
CHEMBL2113474 1 5.83
CHEMBL285819 1 5.72
CHEMBL2113392 1 5.61
CHEMBL443485 5'-Deoxyadenosine 1 5.55
CHEMBL1568 FLUDARABINE 3.0 1 4.98
CHEMBL471144 1 4.96
CHEMBL236757 1 4.77
CHEMBL17857 1 4.71
CHEMBL2079570 1 4.31
CHEMBL278932 1 4.23
CHEMBL202701 1 4.21
CHEMBL279158 1 4.15
CHEMBL3142980 1 4.08
CHEMBL1619 CLADRIBINE 4.0 1 -
CHEMBL17875 1 -
CHEMBL2112597 1 -
CHEMBL416540 1 -
CHEMBL49935 ARISTEROMYCIN 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL119709 IB-MECA Ki = 1.1 nM 8.96
CHEMBL49843 Ki = 16.0 nM 7.80
CHEMBL410873 Ki = 25.6 nM 7.59
CHEMBL464859 NECA Ki = 113.0 nM 6.95
CHEMBL388757 N6-BENZYLADENOSINE Ki = 120.0 nM 6.92
CHEMBL331382 METRIFUDIL Ki = 360.0 nM 6.44
CHEMBL2113395 Ki = 604.0 nM 6.22
CHEMBL2113396 Ki = 703.0 nM 6.15
CHEMBL2113475 Ki = 720.0 nM 6.14
CHEMBL411066 KINETIN RIBOSIDE Ki = 742.0 nM 6.13
CHEMBL2079643 Ki = 1090.0 nM 5.96
CHEMBL2113474 Ki = 1480.0 nM 5.83
CHEMBL285819 Ki = 1890.0 nM 5.72
CHEMBL2113392 Ki = 2480.0 nM 5.61
CHEMBL443485 5'-Deoxyadenosine Ki = 2830.0 nM 5.55
CHEMBL1568 FLUDARABINE Ki = 10400.0 nM 4.98
CHEMBL471144 Ki = 11000.0 nM 4.96
CHEMBL236757 Ki = 16900.0 nM 4.77
CHEMBL17857 Ki = 19500.0 nM 4.71
CHEMBL2079570 Ki = 49300.0 nM 4.31
CHEMBL278932 Ki = 59200.0 nM 4.23
CHEMBL202701 Ki = 61700.0 nM 4.21
CHEMBL279158 Ki = 70400.0 nM 4.15
CHEMBL3142980 Ki = 82400.0 nM 4.08
CHEMBL1619 CLADRIBINE Ki = 207000.0 nM -
CHEMBL17875 Ki = 122000.0 nM -
CHEMBL2112597 Ki = 199000.0 nM -
CHEMBL416540 Ki = 198000.0 nM -
CHEMBL49935 ARISTEROMYCIN Ki = 200000.0 nM -