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Assay Detail

CHEMBL643209

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Displacement of [3H]ZM-241385 from human adenosine A2A receptors expressed in HEK293 cells
12
Total Activities
12
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type HEK293
Confidence 9 — Direct single protein target
Curated By Expert

Target

Adenosine receptor A2a (CHEMBL251)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Pyrazolo[4,3-e]-1,2,4-triazolo[1,5-c]pyrimidine derivatives as adenosine receptor antagonists. Influence of the N5 substituent on the affinity at the human A 3 and A 2B adenosine receptor subtypes: a molecular modeling investigation.
Pastorin G, Da Ros T, Spalluto G, Deflorian F, Moro S, Cacciari B, Baraldi PG, Gessi S, Varani K, Borea PA.
J Med Chem (2003) 46 : 4287 -4296
PubMed 13678407 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
Ki 12 7.24 9.51

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL16867 1 9.51
CHEMBL435022 1 7.42
CHEMBL341777 1 7.40
CHEMBL336217 1 7.38
CHEMBL138593 1 7.35
CHEMBL135719 1 7.26
CHEMBL132806 1 7.24
CHEMBL134566 1 7.22
CHEMBL541455 1 7.22
CHEMBL324735 1 6.42
CHEMBL341376 1 6.37
CHEMBL335950 1 6.10

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL16867 Ki = 0.31 nM 9.51
CHEMBL435022 Ki = 38.0 nM 7.42
CHEMBL341777 Ki = 40.0 nM 7.40
CHEMBL336217 Ki = 42.0 nM 7.38
CHEMBL138593 Ki = 45.0 nM 7.35
CHEMBL135719 Ki = 55.0 nM 7.26
CHEMBL132806 Ki = 57.0 nM 7.24
CHEMBL134566 Ki = 60.0 nM 7.22
CHEMBL541455 Ki = 60.0 nM 7.22
CHEMBL324735 Ki = 381.0 nM 6.42
CHEMBL341376 Ki = 423.0 nM 6.37
CHEMBL335950 Ki = 800.0 nM 6.10