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Assay Detail

CHEMBL643360

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Functional
In vitro inhibition of Acyl coenzyme A:cholesterol acyltransferase in intestinal microsomes isolated from cholesterol-fed rabbits
18
Total Activities
18
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Functional
Organism Oryctolagus cuniculus
Tissue Intestine
Subcellular Microsomes
Confidence 9 — Direct single protein target
Curated By Expert

Target

Acyl-CoA:cholesterol acyltransferase (CHEMBL6141)
Type SINGLE PROTEIN
Organism Oryctolagus cuniculus

Publication

Inhibitors of acyl-CoA:cholesterol acyltransferase. 5. Identification and structure-activity relationships of novel beta-ketoamides as hypocholesterolemic agents.
Augelli-Szafran CE, Blankley CJ, Roth BD, Trivedi BK, Bousley RF, Essenburg AD, Hamelehle KL, Krause BR, Stanfield RL.
J Med Chem (1993) 36 : 2943 -2949
PubMed 8411011 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 18 7.00 8.22

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL102026 1 8.22
CHEMBL316724 1 7.96
CHEMBL102041 1 7.96
CHEMBL98602 1 7.66
CHEMBL98640 1 7.62
CHEMBL101922 1 7.46
CHEMBL431235 1 7.36
CHEMBL99164 1 7.28
CHEMBL98953 1 7.24
CHEMBL318825 1 6.68
CHEMBL101517 1 6.64
CHEMBL98482 1 6.58
CHEMBL101489 1 6.43
CHEMBL99162 1 6.40
CHEMBL317863 1 6.09
CHEMBL420829 1 6.00
CHEMBL98600 1 5.46
CHEMBL98377 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL102026 IC50 = 6.0 nM 8.22
CHEMBL316724 IC50 = 11.0 nM 7.96
CHEMBL102041 IC50 = 11.0 nM 7.96
CHEMBL98602 IC50 = 22.0 nM 7.66
CHEMBL98640 IC50 = 24.0 nM 7.62
CHEMBL101922 IC50 = 35.0 nM 7.46
CHEMBL431235 IC50 = 44.0 nM 7.36
CHEMBL99164 IC50 = 53.0 nM 7.28
CHEMBL98953 IC50 = 57.0 nM 7.24
CHEMBL318825 IC50 = 210.0 nM 6.68
CHEMBL101517 IC50 = 230.0 nM 6.64
CHEMBL98482 IC50 = 260.0 nM 6.58
CHEMBL101489 IC50 = 370.0 nM 6.43
CHEMBL99162 IC50 = 400.0 nM 6.40
CHEMBL317863 IC50 = 810.0 nM 6.09
CHEMBL420829 IC50 = 1000.0 nM 6.00
CHEMBL98600 IC50 = 3500.0 nM 5.46
CHEMBL98377 IC50 > 1000.0 nM -