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Assay Detail

CHEMBL643470

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Binding affinity at adenosine A1 receptor from rat brain membranes by [3H]N6-cyclohexyladenosine displacement.
21
Total Activities
19
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Tissue Brain
Subcellular Membrane
Confidence 8 — Homologous single protein target
Curated By Expert

Target

Adenosine receptor A1 (CHEMBL318)
Type SINGLE PROTEIN
Organism Rattus norvegicus

Publication

N6-[2-(3,5-dimethoxyphenyl)-2-(2-methylphenyl)ethyl]adenosine and its uronamide derivatives. Novel adenosine agonists with both high affinity and high selectivity for the adenosine A2 receptor.
Bridges AJ, Bruns RF, Ortwine DF, Priebe SR, Szotek DL, Trivedi BK.
J Med Chem (1988) 31 : 1282 -1285
PubMed 3385722 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
Ki 21 7.45 9.23

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL68738 N6-CYCLOPENTYLADENOSINE 1 9.23
CHEMBL139000 (R)-PIA 1 8.92
CHEMBL603366 1 8.59
CHEMBL2181971 1 8.28
CHEMBL464859 NECA 1 8.20
CHEMBL193692 1 8.17
CHEMBL604607 1 8.03
CHEMBL604402 1 7.74
CHEMBL132960 1 7.62
CHEMBL606507 1 7.52
CHEMBL606505 1 7.43
CHEMBL603579 1 7.21
CHEMBL2094089 3 6.93
CHEMBL606506 1 6.85
CHEMBL604818 1 6.68
CHEMBL604404 1 6.64
CHEMBL603791 1 6.63
CHEMBL1256672 1 6.22
CHEMBL604819 1 6.00

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL68738 N6-CYCLOPENTYLADENOSINE Ki = 0.59 nM 9.23
CHEMBL139000 (R)-PIA Ki = 1.2 nM 8.92
CHEMBL603366 Ki = 2.6 nM 8.59
CHEMBL2181971 Ki = 5.2 nM 8.28
CHEMBL464859 NECA Ki = 6.3 nM 8.20
CHEMBL193692 Ki = 6.8 nM 8.17
CHEMBL604607 Ki = 9.3 nM 8.03
CHEMBL604402 Ki = 18.4 nM 7.74
CHEMBL132960 Ki = 24.0 nM 7.62
CHEMBL606507 Ki = 30.2 nM 7.52
CHEMBL606505 Ki = 37.0 nM 7.43
CHEMBL603579 Ki = 61.0 nM 7.21
CHEMBL2094089 Ki = 118.0 nM 6.93
CHEMBL606506 Ki = 140.0 nM 6.85
CHEMBL2094089 Ki = 142.0 nM 6.85
CHEMBL2094089 Ki = 188.0 nM 6.73
CHEMBL604818 Ki = 207.0 nM 6.68
CHEMBL604404 Ki = 229.0 nM 6.64
CHEMBL603791 Ki = 232.0 nM 6.63
CHEMBL1256672 Ki = 600.0 nM 6.22
CHEMBL604819 Ki = 1010.0 nM 6.00