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Assay Detail

CHEMBL644125

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
In vitro inhibitory activity against Acyl coenzyme A:cholesterol acyltransferase in intestinal microsomes isolated from cholesterol fed rabbits (IAI).
33
Total Activities
33
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Oryctolagus cuniculus
Tissue Intestine
Subcellular Microsomes
Confidence 9 — Direct single protein target
Curated By Expert

Target

Acyl-CoA:cholesterol acyltransferase (CHEMBL6141)
Type SINGLE PROTEIN
Organism Oryctolagus cuniculus

Publication

Inhibitors of acyl-CoA: cholesterol O-acyl transferase (ACAT) as hypocholesterolemic agents. The synthesis and biological activity of a series of malonester amides
Sliskovic D, Picard J, Roark W, Essenburg A, Krause B, Minton L, Reindel J, Stanfield R
Bioorg Med Chem Lett (1996) 6 : 713 -718
· DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 33 7.10 8.40

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL164479 1 8.40
CHEMBL160103 1 8.40
CHEMBL422709 1 8.22
CHEMBL164205 1 7.96
CHEMBL162996 1 7.96
CHEMBL163293 1 7.92
CHEMBL159666 1 7.92
CHEMBL163325 1 7.82
CHEMBL163615 1 7.82
CHEMBL349364 1 7.57
CHEMBL163562 1 7.46
CHEMBL163237 1 7.42
CHEMBL350852 1 7.38
CHEMBL164203 1 7.28
CHEMBL161109 1 7.28
CHEMBL163356 1 7.17
CHEMBL159285 1 7.15
CHEMBL351882 1 6.89
CHEMBL163860 1 6.68
CHEMBL162831 1 6.58
CHEMBL163763 1 6.50
CHEMBL159722 1 6.09
CHEMBL163688 1 6.00
CHEMBL433717 1 5.77
CHEMBL348070 1 5.75
CHEMBL164260 1 5.21
CHEMBL349908 1 5.19
CHEMBL351272 1 -
CHEMBL162721 1 -
CHEMBL163208 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL164479 IC50 = 4.0 nM 8.40
CHEMBL160103 IC50 = 4.0 nM 8.40
CHEMBL422709 IC50 = 6.0 nM 8.22
CHEMBL164205 IC50 = 11.0 nM 7.96
CHEMBL162996 IC50 = 11.0 nM 7.96
CHEMBL163293 IC50 = 12.0 nM 7.92
CHEMBL159666 IC50 = 12.0 nM 7.92
CHEMBL163325 IC50 = 15.0 nM 7.82
CHEMBL163615 IC50 = 15.0 nM 7.82
CHEMBL349364 IC50 = 27.0 nM 7.57
CHEMBL163562 IC50 = 35.0 nM 7.46
CHEMBL163237 IC50 = 38.0 nM 7.42
CHEMBL350852 IC50 = 42.0 nM 7.38
CHEMBL164203 IC50 = 53.0 nM 7.28
CHEMBL161109 IC50 = 53.0 nM 7.28
CHEMBL163356 IC50 = 67.0 nM 7.17
CHEMBL159285 IC50 = 71.0 nM 7.15
CHEMBL351882 IC50 = 130.0 nM 6.89
CHEMBL163860 IC50 = 210.0 nM 6.68
CHEMBL162831 IC50 = 260.0 nM 6.58
CHEMBL163763 IC50 = 320.0 nM 6.50
CHEMBL159722 IC50 = 810.0 nM 6.09
CHEMBL163688 IC50 = 1000.0 nM 6.00
CHEMBL433717 IC50 = 1700.0 nM 5.77
CHEMBL348070 IC50 = 1800.0 nM 5.75
CHEMBL164260 IC50 = 6200.0 nM 5.21
CHEMBL349908 IC50 = 6400.0 nM 5.19
CHEMBL351272 IC50 < -73.0 % -
CHEMBL162721 IC50 > 5000.0 nM -
CHEMBL163208 IC50 > 5000.0 nM -
CHEMBL163214 IC50 > 5000.0 nM -
CHEMBL163212 IC50 > 5000.0 nM -
CHEMBL262010 IC50 > 5000.0 nM -