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Assay Detail

CHEMBL644670

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Displacement of specific [3H]-SCH- 58261 binding at human Adenosine A2A receptor expressed in HEK293 cells
10
Total Activities
10
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type HEK293
Confidence 9 — Direct single protein target
Curated By Expert

Target

Adenosine receptor A2a (CHEMBL251)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Pyrazolo[4,3-e]-1,2,4-triazolo[1,5-c]pyrimidine derivatives as highly potent and selective human A(3) adenosine receptor antagonists.
Baraldi PG, Cacciari B, Romagnoli R, Spalluto G, Klotz KN, Leung E, Varani K, Gessi S, Merighi S, Borea PA.
J Med Chem (1999) 42 : 4473 -4478
PubMed 10579811 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
Ki 10 7.67 9.74

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL423542 1 9.74
CHEMBL16867 1 9.51
CHEMBL137879 1 8.72
CHEMBL344706 1 8.59
CHEMBL137950 1 6.96
CHEMBL422820 1 6.91
CHEMBL302765 1 6.85
CHEMBL141154 1 6.73
CHEMBL342906 1 6.70
CHEMBL139182 1 5.99

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL423542 Ki = 0.18 nM 9.74
CHEMBL16867 Ki = 0.31 nM 9.51
CHEMBL137879 Ki = 1.9 nM 8.72
CHEMBL344706 Ki = 2.55 nM 8.59
CHEMBL137950 Ki = 110.0 nM 6.96
CHEMBL422820 Ki = 124.0 nM 6.91
CHEMBL302765 Ki = 141.0 nM 6.85
CHEMBL141154 Ki = 185.0 nM 6.73
CHEMBL342906 Ki = 202.0 nM 6.70
CHEMBL139182 Ki = 1015.0 nM 5.99