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Assay Detail

CHEMBL645682

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Intrinsic activity towards Alpha-1 adrenergic receptor by the displacement of [3H]prazosin from rat cerebral cortex membrane in presence of 1 nM GTP.
4
Total Activities
4
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Tissue Cerebral cortex
Confidence 4 — Cell-line / Tissue
Curated By Autocuration

Target

Adrenergic receptor alpha-1 (CHEMBL1907610)
Type PROTEIN FAMILY
Organism Rattus norvegicus

Publication

Alpha1-adrenoceptor antagonists. 6. Structural optimization of pyridazinone-arylpiperazines. Study of the influence on affinity and selectivity of cyclic substituents at the pyridazinone ring and alkoxy groups at the arylpiperazine moiety.
Betti L, Corelli F, Floridi M, Giannaccini G, Maccari L, Manetti F, Strappaghetti G, Botta M.
J Med Chem (2003) 46 : 3555 -3558
PubMed 12877594 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
Ki 4 9.68 10.09

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL47792 1 10.09
CHEMBL300093 1 9.66
CHEMBL2 PRAZOSIN 4.0 1 9.49
CHEMBL117996 1 9.48

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL47792 Ki = 0.081 nM 10.09
CHEMBL300093 Ki = 0.22 nM 9.66
CHEMBL2 PRAZOSIN Ki = 0.32 nM 9.49
CHEMBL117996 Ki = 0.33 nM 9.48