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Assay Detail

CHEMBL645820

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of AGT activity to 50% of control rate in HT-29 cell extract
24
Total Activities
24
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Cell Type HT-29
Confidence 8 — Homologous single protein target
Curated By Expert

Target

Methylated-DNA--protein-cysteine methyltransferase (CHEMBL2864)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Resistance-modifying agents. 8. Inhibition of O(6)-alkylguanine-DNA alkyltransferase by O(6)-alkenyl-, O(6)-cycloalkenyl-, and O(6)-(2-oxoalkyl)guanines and potentiation of temozolomide cytotoxicity in vitro by O(6)-(1-cyclopentenylmethyl)guanine.
Griffin RJ, Arris CE, Bleasdale C, Boyle FT, Calvert AH, Curtin NJ, Dalby C, Kanugula S, Lembicz NK, Newell DR, Pegg AE, Golding BT.
J Med Chem (2000) 43 : 4071 -4083
PubMed 11063604 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 24 5.69 8.52

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL337677 1 8.52
CHEMBL407874 6-O-BENZYLGUANINE 3.0 1 6.75
CHEMBL405259 1 6.41
CHEMBL439608 1 6.26
CHEMBL429409 1 5.80
CHEMBL272182 1 5.58
CHEMBL325053 1 5.07
CHEMBL270982 1 4.80
CHEMBL270483 1 4.70
CHEMBL407876 1 4.60
CHEMBL334050 1 4.11
CHEMBL405197 1 -
CHEMBL272689 1 -
CHEMBL130392 1 -
CHEMBL270945 1 -
CHEMBL272397 1 -
CHEMBL446625 1 -
CHEMBL338451 1 -
CHEMBL406495 1 -
CHEMBL270946 1 -
CHEMBL406419 1 -
CHEMBL337713 1 -
CHEMBL405533 1 -
CHEMBL226395 METHYLGUANINE 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL337677 IC50 = 3.0 nM 8.52
CHEMBL407874 6-O-BENZYLGUANINE IC50 = 180.0 nM 6.75
CHEMBL405259 IC50 = 390.0 nM 6.41
CHEMBL439608 IC50 = 550.0 nM 6.26
CHEMBL429409 IC50 = 1600.0 nM 5.80
CHEMBL272182 IC50 = 2600.0 nM 5.58
CHEMBL325053 IC50 = 8500.0 nM 5.07
CHEMBL270982 IC50 = 16000.0 nM 4.80
CHEMBL270483 IC50 = 20000.0 nM 4.70
CHEMBL407876 IC50 = 25000.0 nM 4.60
CHEMBL334050 IC50 = 77000.0 nM 4.11
CHEMBL405197 IC50 > 1000000.0 nM -
CHEMBL272689 IC50 = 192000.0 nM -
CHEMBL130392 IC50 > 1000000.0 nM -
CHEMBL270945 IC50 > 100000.0 nM -
CHEMBL272397 IC50 > 1000000.0 nM -
CHEMBL446625 IC50 = 548000.0 nM -
CHEMBL338451 IC50 = 554000.0 nM -
CHEMBL406495 IC50 = 1006000.0 nM -
CHEMBL270946 IC50 = 493000.0 nM -
CHEMBL406419 IC50 > 1000000.0 nM -
CHEMBL337713 IC50 = 151000.0 nM -
CHEMBL405533 IC50 > 1000000.0 nM -
CHEMBL226395 METHYLGUANINE IC50 = 428000.0 nM -