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Assay Detail

CHEMBL647467

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Alpha-1-adrenolytic activity was assessed in vitro from the ability to inhibit clonidine binding to rat aorta preparation
16
Total Activities
14
Compounds Tested
2
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Tissue Aorta
Confidence 4 — Cell-line / Tissue
Curated By Autocuration

Target

Adrenergic receptor alpha-1 (CHEMBL1907610)
Type PROTEIN FAMILY
Organism Rattus norvegicus

Publication

New 1,4-dihydropyridine derivatives combining calcium antagonism and alpha-adrenolytic properties.
Marciniak G, Delgado A, Leclerc G, Velly J, Decker N, Schwartz J.
J Med Chem (1989) 32 : 1402 -1407
PubMed 2542561 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 13 4.62 5.00
Kd 3 8.49 10.99

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL2 PRAZOSIN 4.0 1 10.99
CHEMBL597 PHENTOLAMINE 4.0 1 8.01
CHEMBL15245 YOHIMBINE 3.0 1 6.46
CHEMBL3350238 3 5.00
CHEMBL284056 1 5.00
CHEMBL554455 1 4.40
CHEMBL542812 1 4.16
CHEMBL553942 1 -
CHEMBL1484 NICARDIPINE 4.0 1 -
CHEMBL554235 1 -
CHEMBL2093965 1 -
CHEMBL540511 1 -
CHEMBL30911 1 -
CHEMBL542814 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL2 PRAZOSIN Kd = 0.01023 nM 10.99
CHEMBL597 PHENTOLAMINE Kd = 9.772 nM 8.01
CHEMBL15245 YOHIMBINE Kd = 346.74 nM 6.46
CHEMBL3350238 IC50 = 10000.0 nM 5.00
CHEMBL284056 IC50 = 10000.0 nM 5.00
CHEMBL3350238 IC50 = 30000.0 nM 4.52
CHEMBL554455 IC50 = 40000.0 nM 4.40
CHEMBL542812 IC50 = 70000.0 nM 4.16
CHEMBL553942 IC50 >> 100000.0 nM -
CHEMBL1484 NICARDIPINE IC50 > 10000.0 nM -
CHEMBL554235 IC50 > 100000.0 nM -
CHEMBL2093965 IC50 >> 10000.0 nM -
CHEMBL540511 IC50 >> 30000.0 nM -
CHEMBL30911 IC50 > 10000.0 nM -
CHEMBL3350238 IC50 > 30000.0 nM -
CHEMBL542814 IC50 >> 30000.0 nM -