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Assay Detail

CHEMBL648132

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Displacement of [3H]flunitrazepam from diazepam-insensitive GABA-A benzodiazepine receptor of rat cerebellar membrane
13
Total Activities
10
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Confidence 4 — Cell-line / Tissue
Curated By Autocuration

Target

GABA-A receptor; anion channel (CHEMBL1907607)
Type PROTEIN COMPLEX GROUP
Organism Rattus norvegicus

Publication

Synthesis and structure-activity relationships of 3,5-disubstituted 4,5-dihydro-6H-imidazo[1,5-a][1,4]benzodiazepin-6-ones at diazepam-sensitive and diazepam-insensitive benzodiazepine receptors.
Ananthan S, Clayton SD, Ealick SE, Wong G, Evoniuk GE, Skolnick P.
J Med Chem (1993) 36 : 479 -490
PubMed 8386247 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
Ki 13 7.46 8.85

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL52934 2 8.85
CHEMBL6597 Ro-154513 1 8.51
CHEMBL53425 2 8.14
CHEMBL415290 Ro-151310 1 7.77
CHEMBL46547 SARMAZENIL 2.0 1 7.70
CHEMBL54069 1 7.70
CHEMBL301513 1 6.95
CHEMBL145300 1 6.90
CHEMBL300027 2 6.74
CHEMBL267665 RO-147437 1 6.67

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL52934 Ki = 1.4 nM 8.85
CHEMBL6597 Ro-154513 Ki = 3.1 nM 8.51
CHEMBL53425 Ki = 7.3 nM 8.14
CHEMBL52934 Ki = 10.9 nM 7.96
CHEMBL415290 Ro-151310 Ki = 16.9 nM 7.77
CHEMBL46547 SARMAZENIL Ki = 20.0 nM 7.70
CHEMBL54069 Ki = 20.2 nM 7.70
CHEMBL301513 Ki = 112.0 nM 6.95
CHEMBL53425 Ki = 115.0 nM 6.94
CHEMBL145300 Ki = 127.0 nM 6.90
CHEMBL300027 Ki = 184.0 nM 6.74
CHEMBL267665 RO-147437 Ki = 214.0 nM 6.67
CHEMBL300027 Ki = 761.0 nM 6.12