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Assay Detail

CHEMBL649643

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
In vitro inhibitory activity against alpha-2 adrenergic receptor using [3H]clonidine as radioligand in male Sprague-Dawley rats
7
Total Activities
7
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Confidence 4 — Cell-line / Tissue
Curated By Autocuration

Target

Adrenergic receptor alpha-2 (CHEMBL2093864)
Type PROTEIN FAMILY
Organism Rattus norvegicus

Publication

Synthesis, biochemical evaluation, and classical and three-dimensional quantitative structure-activity relationship studies of 7-substituted-1,2,3,4-tetrahydroisoquinolines and their relative affinities toward phenylethanolamine N-methyltransferase and the alpha2-adrenoceptor.
Grunewald GL, Dahanukar VH, Jalluri RK, Criscione KR.
J Med Chem (1999) 42 : 118 -134
PubMed 9888838 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
Ki 7 5.95 6.64

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL281864 1 6.64
CHEMBL14346 1,2,3,4-TETRAHYDROISOQUINOLINE 1 6.46
CHEMBL26711 1 6.44
CHEMBL28329 1 6.39
CHEMBL27499 1 5.89
CHEMBL25316 1 5.33
CHEMBL27599 1 4.47

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL281864 Ki = 230.0 nM 6.64
CHEMBL14346 1,2,3,4-TETRAHYDROISOQUINOLINE Ki = 350.0 nM 6.46
CHEMBL26711 Ki = 360.0 nM 6.44
CHEMBL28329 Ki = 410.0 nM 6.39
CHEMBL27499 Ki = 1300.0 nM 5.89
CHEMBL25316 Ki = 4700.0 nM 5.33
CHEMBL27599 Ki = 34000.0 nM 4.47