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Assay Detail

CHEMBL650877

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of [3H]- flunitrazepam binding to GABA-A Benzodiazepine receptor of rat cerebral cortex membranes
9
Total Activities
9
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Rattus norvegicus
Tissue Cerebral cortex
Subcellular Membrane
Confidence 5 — Multiple protein complex / RNA
Curated By Autocuration

Target

GABA-A receptor; anion channel (CHEMBL1907607)
Type PROTEIN COMPLEX GROUP
Organism Rattus norvegicus

Publication

3-Amino-beta-carboline derivatives and the benzodiazepine receptor. Synthesis of a selective antagonist of the sedative action of diazepam.
Dodd RH, Ouannès C, de Carvalho LP, Valin A, Venault P, Chapouthier G, Rossier J, Potier P.
J Med Chem (1985) 28 : 824 -828
PubMed 2989520 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 9 6.00 8.70

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL11709 1 8.70
CHEMBL165882 1 7.15
CHEMBL353674 1 7.10
CHEMBL353967 1 6.34
CHEMBL167732 1 5.40
CHEMBL353110 1 5.33
CHEMBL167052 1 5.20
CHEMBL54381 1 4.60
CHEMBL165802 1 4.21

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL11709 IC50 = 2.0 nM 8.70
CHEMBL165882 IC50 = 71.0 nM 7.15
CHEMBL353674 IC50 = 80.0 nM 7.10
CHEMBL353967 IC50 = 460.0 nM 6.34
CHEMBL167732 IC50 = 4000.0 nM 5.40
CHEMBL353110 IC50 = 4700.0 nM 5.33
CHEMBL167052 IC50 = 6300.0 nM 5.20
CHEMBL54381 IC50 = 25000.0 nM 4.60
CHEMBL165802 IC50 = 62000.0 nM 4.21