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Assay Detail

CHEMBL652383

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of specific binding of [3H]-BK (1 nM) to rat Bradykinin receptor B2 by 50% in NG108-15 cell membranes
11
Total Activities
11
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Rattus norvegicus
Cell Type NG108-15
Subcellular Membrane
Confidence 9 — Direct single protein target
Curated By Expert

Target

B2 bradykinin receptor (CHEMBL2501)
Type SINGLE PROTEIN
Organism Rattus norvegicus

Publication

Nonpeptide bradykinin B2 receptor antagonists: conversion of rodent-selective bradyzide analogues into potent, orally-active human bradykinin B2 receptor antagonists.
Dziadulewicz EK, Ritchie TJ, Hallett A, Snell CR, Davies JW, Wrigglesworth R, Dunstan AR, Bloomfield GC, Drake GS, McIntyre P, Brown MC, Burgess GM, Lee W, Davis C, Yaqoob M, Phagoo SB, Phillips E, Perkins MN, Campbell EA, Davis AJ, Rang HP.
J Med Chem (2002) 45 : 2160 -2172
PubMed 12014954 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
Ki 11 8.27 10.64

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL1907651 1 10.64
CHEMBL1907652 1 9.51
CHEMBL308468 1 9.30
CHEMBL431562 1 9.22
CHEMBL1907654 1 8.26
CHEMBL1907653 1 8.14
CHEMBL2112283 1 7.97
CHEMBL1907656 1 7.84
CHEMBL2112221 1 7.19
CHEMBL2112220 1 6.75
CHEMBL1907655 1 6.18

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL1907651 Ki = 0.023 nM 10.64
CHEMBL1907652 Ki = 0.31 nM 9.51
CHEMBL308468 Ki = 0.5 nM 9.30
CHEMBL431562 Ki = 0.6 nM 9.22
CHEMBL1907654 Ki = 5.46 nM 8.26
CHEMBL1907653 Ki = 7.3 nM 8.14
CHEMBL2112283 Ki = 10.7 nM 7.97
CHEMBL1907656 Ki = 14.5 nM 7.84
CHEMBL2112221 Ki = 64.5 nM 7.19
CHEMBL2112220 Ki = 176.0 nM 6.75
CHEMBL1907655 Ki = 654.0 nM 6.18