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Assay Detail

CHEMBL652568

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Functional
Growth inhibition of CCRF-CEM human leukemic lymphoblasts
15
Total Activities
15
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Functional
Organism Homo sapiens
Cell Type CCRF-CEM
Confidence 1 — Organism
Curated By Expert

Target

CCRF-CEM (CHEMBL382)
Type CELL-LINE
Organism Homo sapiens

Publication

Analogues of the potent nonpolyglutamatable antifolate N(alpha)-(4-amino-4-deoxypteroyl)-N(delta)-hemiphthaloyl-L-ornithine (PT523) with modifications in the side chain, p-aminobenzoyl moiety, or 9,10-bridge: synthesis and in vitro antitumor activity.
Rosowsky A, Wright JE, Vaidya CM, Forsch RA, Bader H.
J Med Chem (2000) 43 : 1620 -1634
PubMed 10780919 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 15 8.43 9.28

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL297566 1 9.28
CHEMBL298032 1 9.20
CHEMBL42141 1 8.92
CHEMBL275023 1 8.92
CHEMBL18155 1 8.82
CHEMBL44919 1 8.54
CHEMBL296373 EDATREXATE 2.0 1 8.48
CHEMBL42889 1 8.36
CHEMBL376180 AMINOPTERIN 2.0 1 8.36
CHEMBL290590 1 8.30
CHEMBL43150 1 8.29
CHEMBL42819 1 8.26
CHEMBL42142 1 8.12
CHEMBL42888 1 7.27
CHEMBL42109 1 7.26

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL297566 IC50 = 0.53 nM 9.28
CHEMBL298032 IC50 = 0.63 nM 9.20
CHEMBL42141 IC50 = 1.2 nM 8.92
CHEMBL275023 IC50 = 1.2 nM 8.92
CHEMBL18155 IC50 = 1.5 nM 8.82
CHEMBL44919 IC50 = 2.9 nM 8.54
CHEMBL296373 EDATREXATE IC50 = 3.3 nM 8.48
CHEMBL42889 IC50 = 4.4 nM 8.36
CHEMBL376180 AMINOPTERIN IC50 = 4.4 nM 8.36
CHEMBL290590 IC50 = 5.0 nM 8.30
CHEMBL43150 IC50 = 5.1 nM 8.29
CHEMBL42819 IC50 = 5.5 nM 8.26
CHEMBL42142 IC50 = 7.5 nM 8.12
CHEMBL42888 IC50 = 54.0 nM 7.27
CHEMBL42109 IC50 = 55.0 nM 7.26