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Assay Detail

CHEMBL654240

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of [3H]flunitrazepam binding to GABA-A central Benzodiazepine receptor of bovine brain membranes
16
Total Activities
16
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Bos taurus
Subcellular Brain membranes
Confidence 5 — Multiple protein complex / RNA
Curated By Autocuration

Target

GABA-A receptor; anion channel (CHEMBL2094107)
Type PROTEIN COMPLEX GROUP
Organism Bos taurus

Publication

Specific inhibition of benzodiazepine receptor binding by some N-(indol-3-ylglyoxylyl)amino acid derivatives.
Martini C, Gervasio T, Lucacchini A, Da Settimo A, Primofiore G, Marini AM.
J Med Chem (1985) 28 : 506 -509
PubMed 2984422 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 16 5.38 7.42

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL13209 NITRAZEPAM 3.0 1 7.42
CHEMBL12 DIAZEPAM 4.0 1 7.39
CHEMBL314045 1 6.70
CHEMBL84859 1 6.30
CHEMBL451 CHLORDIAZEPOXIDE 4.0 1 5.96
CHEMBL147766 1 5.70
CHEMBL85966 1 5.32
CHEMBL148451 1 5.27
CHEMBL82672 1 5.21
CHEMBL146364 1 4.70
CHEMBL146082 1 4.66
CHEMBL82675 1 4.66
CHEMBL146415 1 4.42
CHEMBL146094 1 4.22
CHEMBL145854 1 4.12
CHEMBL148040 1 4.10

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL13209 NITRAZEPAM IC50 = 38.0 nM 7.42
CHEMBL12 DIAZEPAM IC50 = 41.0 nM 7.39
CHEMBL314045 IC50 = 200.0 nM 6.70
CHEMBL84859 IC50 = 500.0 nM 6.30
CHEMBL451 CHLORDIAZEPOXIDE IC50 = 1100.0 nM 5.96
CHEMBL147766 IC50 = 2000.0 nM 5.70
CHEMBL85966 IC50 = 4800.0 nM 5.32
CHEMBL148451 IC50 = 5400.0 nM 5.27
CHEMBL82672 IC50 = 6200.0 nM 5.21
CHEMBL146364 IC50 = 20000.0 nM 4.70
CHEMBL146082 IC50 = 22000.0 nM 4.66
CHEMBL82675 IC50 = 22000.0 nM 4.66
CHEMBL146415 IC50 = 38000.0 nM 4.42
CHEMBL146094 IC50 = 60000.0 nM 4.22
CHEMBL145854 IC50 = 75000.0 nM 4.12
CHEMBL148040 IC50 = 80000.0 nM 4.10