Assay Detail
Functional
CHEMBL656266
Review assay metadata, readout intent, target linkage, and publication context from the same page.
In vitro cytotoxicity by its growth inhibitory activity against human leukemic lymphoblasts (CEM cells).
8
Total Activities
8
Compounds Tested
1
Activity Types
0
Assay Parameters
Assay Information
| Assay Type | Functional |
| Organism | Homo sapiens |
| Cell Type | CCRF-CEM |
| Confidence | 1 — Organism |
| Curated By | Intermediate |
Publication
Methotrexate analogues. 21. Divergent influence of alkyl chain length on the dihydrofolate reductase affinity and cytotoxicity of methotrexate monoesters.
Activity Statistics
| Type | Count | Avg pChEMBL | Best pChEMBL |
|---|---|---|---|
| IC50 | 8 | 6.37 | 7.60 |
Compounds Tested
| Compound | Name | Phase | Activities | Best pChEMBL |
|---|---|---|---|---|
| CHEMBL34259 | METHOTREXATE | 4.0 | 1 | 7.60 |
| CHEMBL351766 | — | — | 1 | 6.96 |
| CHEMBL157083 | — | — | 1 | 6.60 |
| CHEMBL351765 | — | — | 1 | 6.43 |
| CHEMBL155016 | — | — | 1 | 6.11 |
| CHEMBL154152 | — | — | 1 | 6.04 |
| CHEMBL157904 | — | — | 1 | 5.70 |
| CHEMBL347439 | — | — | 1 | 5.52 |
Activity Data
| Compound | Name | Type | Rel. | Value | Units | pChEMBL |
|---|---|---|---|---|---|---|
| CHEMBL34259 | METHOTREXATE | IC50 | = | 25.0 | nM | 7.60 |
| CHEMBL351766 | — | IC50 | = | 110.0 | nM | 6.96 |
| CHEMBL157083 | — | IC50 | = | 250.0 | nM | 6.60 |
| CHEMBL351765 | — | IC50 | = | 370.0 | nM | 6.43 |
| CHEMBL155016 | — | IC50 | = | 780.0 | nM | 6.11 |
| CHEMBL154152 | — | IC50 | = | 920.0 | nM | 6.04 |
| CHEMBL157904 | — | IC50 | = | 2000.0 | nM | 5.70 |
| CHEMBL347439 | — | IC50 | = | 3000.0 | nM | 5.52 |