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Assay Detail

CHEMBL658555

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Functional
Inhibitory activity to reduce cell density by 50% of AA8 cells under aerobic conditions
9
Total Activities
9
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Functional
Organism Cricetulus griseus
Cell Type CHO-AA8
Confidence 1 — Organism
Curated By Intermediate

Target

CHO-AA8 (CHEMBL614555)
Type CELL-LINE
Organism Cricetulus griseus

Publication

Hypoxia-selective antitumor agents. 2. Electronic effects of 4-substituents on the mechanisms of cytotoxicity and metabolic stability of nitracrine derivatives.
Wilson WR, Thompson LH, Anderson RF, Denny WA.
J Med Chem (1989) 32 : 31 -38
PubMed 2909741 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 9 5.98 7.58

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL26291 NITRACRINE 2.0 1 7.58
CHEMBL103637 1 7.48
CHEMBL312975 1 7.12
CHEMBL103310 1 6.60
CHEMBL312249 1 6.21
CHEMBL103914 1 5.54
CHEMBL317248 1 4.72
CHEMBL101478 1 4.37
CHEMBL421212 1 4.21

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL26291 NITRACRINE IC50 = 26.3 nM 7.58
CHEMBL103637 IC50 = 33.0 nM 7.48
CHEMBL312975 IC50 = 76.0 nM 7.12
CHEMBL103310 IC50 = 250.0 nM 6.60
CHEMBL312249 IC50 = 616.0 nM 6.21
CHEMBL103914 IC50 = 2900.0 nM 5.54
CHEMBL317248 IC50 = 18900.0 nM 4.72
CHEMBL101478 IC50 = 43000.0 nM 4.37
CHEMBL421212 IC50 = 62000.0 nM 4.21