Assay Detail
Functional
CHEMBL663892
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Inhibitory activity against dihydrofolate reductase in Lactobacillus casei was determined (glutamate residue 2)
6
Total Activities
3
Compounds Tested
1
Activity Types
0
Assay Parameters
Assay Information
| Assay Type | Functional |
| Organism | Lacticaseibacillus casei |
| Confidence | 9 — Direct single protein target |
| Curated By | Autocuration |
Publication
Synthesis and biological evaluation of poly-gamma-glutamyl metabolites of 10-deazaaminopterin and 10-ethyl-10-deazaaminopterin.
Activity Statistics
| Type | Count | Avg pChEMBL | Best pChEMBL |
|---|---|---|---|
| IC50 | 6 | 7.75 | 8.05 |
Compounds Tested
| Compound | Name | Phase | Activities | Best pChEMBL |
|---|---|---|---|---|
| CHEMBL296373 | EDATREXATE | 2.0 | 2 | 8.05 |
| CHEMBL34259 | METHOTREXATE | 4.0 | 2 | 7.80 |
| CHEMBL293263 | — | — | 2 | 7.75 |
Activity Data
| Compound | Name | Type | Rel. | Value | Units | pChEMBL |
|---|---|---|---|---|---|---|
| CHEMBL296373 | EDATREXATE | IC50 | = | 9.0 | nM | 8.05 |
| CHEMBL34259 | METHOTREXATE | IC50 | = | 16.0 | nM | 7.80 |
| CHEMBL293263 | — | IC50 | = | 18.0 | nM | 7.75 |
| CHEMBL296373 | EDATREXATE | IC50 | = | 21.0 | nM | 7.68 |
| CHEMBL293263 | — | IC50 | = | 24.0 | nM | 7.62 |
| CHEMBL34259 | METHOTREXATE | IC50 | = | 26.0 | nM | 7.58 |