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Assay Detail

CHEMBL664717

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibitory concentration for DHFR in Lactobacillus casei
7
Total Activities
7
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Lacticaseibacillus casei
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Dihydrofolate reductase (CHEMBL2902)
Type SINGLE PROTEIN
Organism Lactobacillus casei

Publication

Classical and nonclassical furo[2,3-d]pyrimidines as novel antifolates: synthesis and biological activities.
Gangjee A, Devraj R, McGuire JJ, Kisliuk RL, Queener SF, Barrows LR.
J Med Chem (1994) 37 : 1169 -1176
PubMed 8164259 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 7 7.04 8.22

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL34259 METHOTREXATE 4.0 1 8.22
CHEMBL278515 1 6.66
CHEMBL17383 1 6.25
CHEMBL274278 1 -
CHEMBL16950 1 -
CHEMBL17044 1 -
CHEMBL17235 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL34259 METHOTREXATE IC50 = 6.0 nM 8.22
CHEMBL278515 IC50 = 220.0 nM 6.66
CHEMBL17383 IC50 = 560.0 nM 6.25
CHEMBL274278 IC50 > 3000.0 nM -
CHEMBL16950 IC50 > 26000.0 nM -
CHEMBL17044 IC50 > 31000.0 nM -
CHEMBL17235 IC50 > 25000.0 nM -