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Assay Detail

CHEMBL664859

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Binding
Binding affinity against Dihydrofolate reductase of Escherichia coli
18
Total Activities
18
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Escherichia coli
Confidence 9 — Direct single protein target
Curated By Intermediate

Target

Dihydrofolate reductase (CHEMBL1809)
Type SINGLE PROTEIN
Organism Escherichia coli

Publication

Receptor-based design of dihydrofolate reductase inhibitors: comparison of crystallographically determined enzyme binding with enzyme affinity in a series of carboxy-substituted trimethoprim analogues.
Kuyper LF, Roth B, Baccanari DP, Ferone R, Beddell CR, Champness JN, Stammers DK, Dann JG, Norrington FE, Baker DJ.
J Med Chem (1985) 28 : 303 -311
PubMed 3973902 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
Ki 18 9.16 10.68

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL34259 METHOTREXATE 4.0 1 10.68
CHEMBL14001 1 10.62
CHEMBL418347 1 10.46
CHEMBL274430 1 10.30
CHEMBL13673 1 10.18
CHEMBL13820 1 9.43
CHEMBL14002 1 9.33
CHEMBL13877 1 9.12
CHEMBL22 TRIMETHOPRIM 4.0 1 8.89
CHEMBL13908 1 8.72
CHEMBL321942 1 8.66
CHEMBL104369 1 8.64
CHEMBL14201 1 8.59
CHEMBL322001 1 8.57
CHEMBL105006 1 8.51
CHEMBL105283 1 8.39
CHEMBL318078 1 8.01
CHEMBL104675 1 7.80

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL34259 METHOTREXATE Ki = 0.021 nM 10.68
CHEMBL14001 Ki = 0.024 nM 10.62
CHEMBL418347 Ki = 0.035 nM 10.46
CHEMBL274430 Ki = 0.05 nM 10.30
CHEMBL13673 Ki = 0.066 nM 10.18
CHEMBL13820 Ki = 0.37 nM 9.43
CHEMBL14002 Ki = 0.47 nM 9.33
CHEMBL13877 Ki = 0.76 nM 9.12
CHEMBL22 TRIMETHOPRIM Ki = 1.3 nM 8.89
CHEMBL13908 Ki = 1.9 nM 8.72
CHEMBL321942 Ki = 2.2 nM 8.66
CHEMBL104369 Ki = 2.3 nM 8.64
CHEMBL14201 Ki = 2.6 nM 8.59
CHEMBL322001 Ki = 2.7 nM 8.57
CHEMBL105006 Ki = 3.1 nM 8.51
CHEMBL105283 Ki = 4.1 nM 8.39
CHEMBL318078 Ki = 9.8 nM 8.01
CHEMBL104675 Ki = 16.0 nM 7.80