Assay Detail
Binding
CHEMBL666732
Review assay metadata, readout intent, target linkage, and publication context from the same page.
Compound was evaluated as inhibitor of Escherichia coli Dihydrofolate reductase
6
Total Activities
6
Compounds Tested
1
Activity Types
0
Assay Parameters
Assay Information
| Assay Type | Binding |
| Organism | Escherichia coli |
| Confidence | 9 — Direct single protein target |
| Curated By | Intermediate |
Publication
Design, synthesis, and biological activities of classical N-[4-[2-(2-amino-4-ethylpyrrolo[2,3-d]pyrimidin-5-yl)ethyl]benzoyl]-l-glutamic acid and its 6-methyl derivative as potential dual inhibitors of thymidylate synthase and dihydrofolate reductase and as potential antitumor agents.
Activity Statistics
| Type | Count | Avg pChEMBL | Best pChEMBL |
|---|---|---|---|
| IC50 | 6 | 6.48 | 8.15 |
Compounds Tested
| Compound | Name | Phase | Activities | Best pChEMBL |
|---|---|---|---|---|
| CHEMBL34259 | METHOTREXATE | 4.0 | 1 | 8.15 |
| CHEMBL22 | TRIMETHOPRIM | 4.0 | 1 | 7.82 |
| CHEMBL157434 | — | — | 1 | 6.04 |
| CHEMBL439973 | — | — | 1 | 5.75 |
| CHEMBL154935 | — | — | 1 | 4.66 |
| CHEMBL225072 | PEMETREXED | 4.0 | 1 | - |
Activity Data
| Compound | Name | Type | Rel. | Value | Units | pChEMBL |
|---|---|---|---|---|---|---|
| CHEMBL34259 | METHOTREXATE | IC50 | = | 7.0 | nM | 8.15 |
| CHEMBL22 | TRIMETHOPRIM | IC50 | = | 15.0 | nM | 7.82 |
| CHEMBL157434 | — | IC50 | = | 920.0 | nM | 6.04 |
| CHEMBL439973 | — | IC50 | = | 1800.0 | nM | 5.75 |
| CHEMBL154935 | — | IC50 | = | 22000.0 | nM | 4.66 |
| CHEMBL225072 | PEMETREXED | IC50 | = | 230000.0 | nM | - |