Assay Detail
Binding
CHEMBL667167
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Thermodynamic Dissociation Constant for compound-Val31-dihydrofolate reductase (DHFR) complex at pH 7
4
Total Activities
4
Compounds Tested
1
Activity Types
0
Assay Parameters
Assay Information
| Assay Type | Binding |
| Organism | Escherichia coli |
| Confidence | 9 — Direct single protein target |
| Curated By | Intermediate |
Publication
Evaluation of the importance of hydrophobic interactions in drug binding to dihydrofolate reductase.
Activity Statistics
| Type | Count | Avg pChEMBL | Best pChEMBL |
|---|---|---|---|
| Kd | 4 | 5.92 | 6.66 |
Compounds Tested
| Compound | Name | Phase | Activities | Best pChEMBL |
|---|---|---|---|---|
| CHEMBL2364573 | NICOTINAMIDE ADENINE DINUCLEOTIDE PHOSPHATE | — | 1 | 6.66 |
| CHEMBL2021342 | — | — | 1 | 6.52 |
| CHEMBL1622 | FOLIC ACID | 4.0 | 1 | 5.32 |
| CHEMBL46294 | DIHYDROFOLIC ACID | — | 1 | 5.18 |
Activity Data
| Compound | Name | Type | Rel. | Value | Units | pChEMBL |
|---|---|---|---|---|---|---|
| CHEMBL2364573 | NICOTINAMIDE ADENINE DINUCLEOTIDE PHOSPHATE | Kd | = | 220.0 | nM | 6.66 |
| CHEMBL2021342 | — | Kd | = | 300.0 | nM | 6.52 |
| CHEMBL1622 | FOLIC ACID | Kd | = | 4800.0 | nM | 5.32 |
| CHEMBL46294 | DIHYDROFOLIC ACID | Kd | = | 6600.0 | nM | 5.18 |