Assay Detail
Binding
CHEMBL667171
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Thermodynamic dissociation constant of compound for mutant T46A Escherichia coli dihydrofolate reductase
2
Total Activities
2
Compounds Tested
1
Activity Types
0
Assay Parameters
Assay Information
| Assay Type | Binding |
| Organism | Escherichia coli |
| Confidence | 9 — Direct single protein target |
| Curated By | Intermediate |
Publication
Probing the molecular basis of resistance to pyrimethamine by site-directed mutagenesis.
Activity Statistics
| Type | Count | Avg pChEMBL | Best pChEMBL |
|---|---|---|---|
| Kd | 2 | 7.73 | 8.85 |
Compounds Tested
| Compound | Name | Phase | Activities | Best pChEMBL |
|---|---|---|---|---|
| CHEMBL36 | PYRIMETHAMINE | 4.0 | 1 | 8.85 |
| CHEMBL46294 | DIHYDROFOLIC ACID | — | 1 | 6.61 |
Activity Data
| Compound | Name | Type | Rel. | Value | Units | pChEMBL |
|---|---|---|---|---|---|---|
| CHEMBL36 | PYRIMETHAMINE | Kd | = | 1.4 | nM | 8.85 |
| CHEMBL46294 | DIHYDROFOLIC ACID | Kd | = | 243.0 | nM | 6.61 |