Assay Detail
Binding
CHEMBL667228
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Inhibitory activity against dihydrofolate reductase (DHFR) enzyme from rat liver
9
Total Activities
9
Compounds Tested
1
Activity Types
0
Assay Parameters
Assay Information
| Assay Type | Binding |
| Confidence | 8 — Homologous single protein target |
| Curated By | Autocuration |
Publication
2,4-Diamino-5-benzylpyrimidines as antibacterial agents. 8. The 3,4,5-triethyl isostere of trimethoprim. A study of specificity.
Activity Statistics
| Type | Count | Avg pChEMBL | Best pChEMBL |
|---|---|---|---|
| IC50 | 9 | 4.33 | 4.54 |
Compounds Tested
| Compound | Name | Phase | Activities | Best pChEMBL |
|---|---|---|---|---|
| CHEMBL60883 | — | — | 1 | 4.54 |
| CHEMBL57582 | — | — | 1 | 4.42 |
| CHEMBL293013 | — | — | 1 | 4.23 |
| CHEMBL19633 | DIAVERIDINE | 2.0 | 1 | 4.11 |
| CHEMBL19023 | — | — | 1 | - |
| CHEMBL22 | TRIMETHOPRIM | 4.0 | 1 | - |
| CHEMBL61287 | — | — | 1 | - |
| CHEMBL59703 | — | — | 1 | - |
| CHEMBL60029 | — | — | 1 | - |
Activity Data
| Compound | Name | Type | Rel. | Value | Units | pChEMBL |
|---|---|---|---|---|---|---|
| CHEMBL60883 | — | IC50 | = | 29000.0 | nM | 4.54 |
| CHEMBL57582 | — | IC50 | = | 38000.0 | nM | 4.42 |
| CHEMBL293013 | — | IC50 | = | 59000.0 | nM | 4.23 |
| CHEMBL19633 | DIAVERIDINE | IC50 | = | 78000.0 | nM | 4.11 |
| CHEMBL19023 | — | IC50 | = | 170000.0 | nM | - |
| CHEMBL22 | TRIMETHOPRIM | IC50 | = | 260000.0 | nM | - |
| CHEMBL61287 | — | IC50 | = | 210000.0 | nM | - |
| CHEMBL59703 | — | IC50 | = | 230000.0 | nM | - |
| CHEMBL60029 | — | IC50 | > | 40000.0 | nM | - |