Skip to main content
Free research Free research Free assay review with research home and workspace preview
Quick search ChEMBL 36
Assay Detail

CHEMBL667768

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Tested for inhibitory activity against dihydrofolate reductase in Beef liver
7
Total Activities
7
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Bos taurus
Confidence 8 — Homologous single protein target
Curated By Autocuration

Target

Dihydrofolate reductase (CHEMBL2902)
Type SINGLE PROTEIN
Organism Lactobacillus casei

Publication

2,4-Diaminothieno[2,3-d]pyrimidine analogues of trimetrexate and piritrexim as potential inhibitors of Pneumocystis carinii and Toxoplasma gondii dihydrofolate reductase.
Rosowsky A, Mota CE, Wright JE, Freisheim JH, Heusner JJ, McCormack JJ, Queener SF.
J Med Chem (1993) 36 : 3103 -3112
PubMed 8230096 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 7 5.81 6.70

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL104770 1 6.70
CHEMBL108006 1 6.40
CHEMBL320811 1 6.19
CHEMBL104555 1 6.19
CHEMBL107167 1 5.34
CHEMBL107079 1 5.11
CHEMBL321826 1 4.72

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL104770 IC50 = 200.0 nM 6.70
CHEMBL108006 IC50 = 400.0 nM 6.40
CHEMBL320811 IC50 = 640.0 nM 6.19
CHEMBL104555 IC50 = 650.0 nM 6.19
CHEMBL107167 IC50 = 4600.0 nM 5.34
CHEMBL107079 IC50 = 7700.0 nM 5.11
CHEMBL321826 IC50 = 19000.0 nM 4.72