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Assay Detail

CHEMBL667808

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibitory activity against Dihydrofolate reductase from Lactobacillus casei
7
Total Activities
7
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Lacticaseibacillus casei
Confidence 9 — Direct single protein target
Curated By Intermediate

Target

Dihydrofolate reductase (CHEMBL2902)
Type SINGLE PROTEIN
Organism Lactobacillus casei

Publication

6-substituted 2,4-diamino-5-methylpyrido[2,3-d]pyrimidines as inhibitors of dihydrofolate reductases from Pneumocystis carinii and Toxoplasma gondii and as antitumor agents.
Gangjee A, Vasudevan A, Queener SF, Kisliuk RL.
J Med Chem (1995) 38 : 1778 -1785
PubMed 7752201 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 7 7.16 7.66

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL23532 1 7.66
CHEMBL119 TRIMETREXATE 4.0 1 7.57
CHEMBL23021 1 7.41
CHEMBL283518 1 7.08
CHEMBL22 TRIMETHOPRIM 4.0 1 6.99
CHEMBL50514 1 6.70
CHEMBL53693 1 6.68

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL23532 IC50 = 22.0 nM 7.66
CHEMBL119 TRIMETREXATE IC50 = 27.0 nM 7.57
CHEMBL23021 IC50 = 39.0 nM 7.41
CHEMBL283518 IC50 = 83.0 nM 7.08
CHEMBL22 TRIMETHOPRIM IC50 = 103.0 nM 6.99
CHEMBL50514 IC50 = 200.0 nM 6.70
CHEMBL53693 IC50 = 210.0 nM 6.68